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      FRET-Based Mito-Specific Fluorescent Probe for Ratiometric Detection and Imaging of Endogenous Peroxynitrite: Dyad of Cy3 and Cy5.

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          Abstract

          Peroxynitrite (OONO(-)) is profoundly implicated in health and disease. The physiological and pathological outcome of OONO(-) is related to its local concentration, and hence, a reliable OONO(-) assay is highly desired. We have developed a FRET-based small-molecule fluorescent probe (PNCy3Cy5), harnessing the differential reactivity of Cy3 and Cy5 toward OONO(-) by fine-tuning. It exhibits high detection sensitivity and yields a ratiometric fluorescent signal. We have exemplified that it can be applied in semiquantitative determination of OONO(-) in living cells. Notably, it specifically localizes in mitochondria, where endogenous OONO(-) is predominantly generated. Thus, PNCy3Cy5 is a promising molecular tool for peroxynitrite biology.

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          Author and article information

          Journal
          J. Am. Chem. Soc.
          Journal of the American Chemical Society
          American Chemical Society (ACS)
          1520-5126
          0002-7863
          August 31 2016
          : 138
          : 34
          Affiliations
          [1 ] State Key Laboratory of Bioreactor Engineering, Shanghai Key Laboratory of Chemical Biology, and ‡Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology , Meilong Road 130, Shanghai 200237, China.
          Article
          10.1021/jacs.6b06398
          27517310
          c3613ee5-eece-4b94-af71-8c1d9d0653d2
          History

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