Magnetocontrollability of Fe7C3@C superparamagnetic nanoparticles in living cells

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Abstract

Background

A new type of superparamagnetic nanoparticles with chemical formula Fe7C3@C (MNPs) showed higher value of magnetization compared to traditionally used iron oxide-based nanoparticles as was shown in our previous studies. The in vitro biocompatibility tests demonstrated that the MNPs display high efficiency of cellular uptake and do not affect cyto-physiological parameters of cultured cells. These MNPs display effective magnetocontrollability in homogeneous liquids but their behavior in cytoplasm of living cells under the effect of magnetic field was not carefully analyzed yet.

Results

In this work we investigated the magnetocontrollability of MNPs interacting with living cells in permanent magnetic field. It has been shown that cells were capable of capturing MNPs by upper part of the cell membrane, and from the surface of the cultivation substrate during motion process. Immunofluorescence studies using intracellular endosomal membrane marker showed that MNP agglomerates can be either located in endosomes or lying free in the cytoplasm. When attached cells were exposed to a magnetic field up to 0.15 T, the MNPs acquired magnetic moment and the displacement of incorporated MNP agglomerates in the direction of the magnet was observed. Weakly attached or non-attached cells, such as cells in mitosis or after cytoskeleton damaging treatments moved towards the magnet. During long time cultivation of cells with MNPs in a magnetic field gradual clearing of cells from MNPs was observed. It was the result of removing MNPs from the surface of the cell agglomerates discarded in the process of exocytosis.

Conclusions

Our data allow us to conclude for the first time that the magnetic properties of the MNPs are sufficient for successful manipulation with MNP agglomerates both at the intracellular level, and within the whole cell. The structure of the outer shells of the MNPs allows firmly associate different types of biological molecules with them. This creates prospects for the use of such complexes for targeted delivery and selective removal of selected biological molecules from living cells.

Electronic supplementary material

The online version of this article (doi:10.1186/s12951-016-0219-4) contains supplementary material, which is available to authorized users.

Related collections

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Author and article information

Contributors

Irina B. Alieva: irina_alieva@mail.ru

Igor Kireev: kireev@genebee.msu.ru

Anastasia S. Garanina: anastasiacit@gmail.com

Natalia Alyabyeva: alyabyeva@univ-tours.fr

Antoine Ruyter: antoine.ruyter@univ-tours.fr

Olga S. Strelkova: olgastrelkova@gmail.com

Oxana A. Zhironkina: oxana.zhironkina@gmail.com

Varvara D. Cherepaninets: vvvvvdddd@gmail.com

Alexander G. Majouga: alexander.majouga@gmail.com

Valery A. Davydov: vdavydov@hppi.troitsk.ru

Valery N. Khabashesku: Valery.Khabashesku@bakerhughes.com

Viatcheslav Agafonov: viatcheslav.agafonov@univ-tours.fr

Rustem E. Uzbekov:

ORCID: http://orcid.org/0000-0002-9336-5484

rustem.uzbekov@univ-tours.fr

Journal

Journal ID (nlm-ta): J Nanobiotechnology

Journal ID (iso-abbrev): J Nanobiotechnology

Title: Journal of Nanobiotechnology

Publisher: BioMed Central (London )

ISSN (Electronic): 1477-3155

Publication date (Electronic): 30 August 2016

Publication date PMC-release: 30 August 2016

Publication date Collection: 2016

Volume: 14

Issue: 1

Electronic Location Identifier: 67

Affiliations

[1 ]A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Moscow, Russia 119992

[2 ]Biology Faculty, Moscow State University, Moscow, Russia 119992

[3 ]GREMAN, UMR CNRS 7347, Université François Rabelais, 37200 Tours, France

[4 ]Chemistry Faculty, Moscow State University, Moscow, Russia 119992

[5 ]MISiS, Leninskiy prospekt 2, Moscow, Russia 119049

[6 ]Institute of High Pressure Physics RAS, Troitsk, Moscow region Russia 142190

[7 ]Center for Technology Innovation, Baker Hughes Inc., Houston, TX 77040 USA

[8 ]Laboratoire Biologie Cellulaire et Microscopie Electronique, Faculté de Médecine, Université François Rabelais, 37032 Tours, France

[9 ]Faculty of Bioengineering and Bioinformatics, Moscow State University, Moscow, Russia 119992

Author information

Rustem E. Uzbekov http://orcid.org/0000-0002-9336-5484

Article

Publisher ID: 219

DOI: 10.1186/s12951-016-0219-4

PMC ID: 5006615

PubMed ID: 27576904

SO-VID: c38efde8-0806-4ec1-b1f0-392d1252ea08

License:

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License ( http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver ( http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

History

Date received : 27 April 2016

Date accepted : 18 August 2016

Funding

Funded by: Russian Foundation for Basic Research (RU)

Award ID: 15-03-04490

Award Recipient : Valery A. Davydov

Funded by: FundRef http://dx.doi.org/10.13039/501100002261, Russian Foundation for Basic Research;

Award ID: 15-04-08550

Award ID: 15-54-77078

Award Recipient : Irina B. Alieva Igor Kireev

Funded by: Lomonosov Moscow State University Development Program

Award ID: PNR 5.13

Award Recipient : Igor Kireev

Funded by: The NUST MISiS grant designed to invite Leading scientists for short term joined research projects

Custom metadata

ScienceOpen disciplines: Biotechnology

Keywords: superparamagnetic nanoparticles,living cells,magnetocontrollability,endocytosis,cytoskeleton,cell adhesion

Data availability:

ScienceOpen disciplines: Biotechnology

Keywords: superparamagnetic nanoparticles, living cells, magnetocontrollability, endocytosis, cytoskeleton, cell adhesion