Interaction of stomatal behaviour and vulnerability to xylem cavitation determines the drought response of three temperate tree species

Abstract

How the mortality and growth of tree species vary with the iso-anisohydric continuum and xylem vulnerability is still being debated. We conducted a precipitation reduction experiment to create a mild drought condition in a forest in the Baotianman Mountains, China, a sub-humid region. Three main sub-canopy tree species in this region were examined. After rainfall reduction, Lindera obtusiloba showed severe dieback, but two other co-occurring species did not show dieback. The water potential at stomatal closure of Dendrobenthamia japonica, L. obtusiloba and Sorbus alnifolia was −1.70, −2.54 and −3.41 MPa, respectively, whereas the water potential at 88 % loss in hydraulic conductivity of the three species was −2.31, −2.11 and −7.01 MPa, respectively. Taken together, near-anisohydric L. obtusiloba with vulnerable xylem was highly susceptible to drought dieback. Anisohydric S. alnifolia had the most negative minimum water potential, and its xylem was the most resistant to cavitation. Isohydric D. japonica conserved water by rapidly closing its stomata. Ultimately, the hydraulic safety margin (HSM) of L. obtusiloba was the smallest among the three species, especially in precipitation-reduced plots. In terms of the stomatal safety margin (SSM), L. obtusiloba was negative, while S. alnifolia and D. japonica were positive. Of the two species without dieback, rainfall reduction decreased growth of D. japonica, but did not influence growth of S. Alnifolia; meanwhile, rainfall reduction led to a decrease of non-structural carbohydrates (NSCs) in D. japonica, but an increase in S. alnifolia. It is concluded that HSM as well as SSM allow interpreting the sensitivity of the three sub-canopy species to drought. The drought-induced dieback of L. obtusiloba is determined by the interaction of stomatal behaviour and xylem vulnerability, and the species could be sensitive to climate change-caused drought although still in sub-humid areas. The isohydric/anisohydric degree is associated with NSCs status and growth of plants.

Abstract

Climate change-induced drought has caused increasing plant mortality around the globe. In this study, we artificially reduced annual rainfall in the field of temperate continental mountains, and investigated different responses of three understory tree species to drought and their response strategies. This study aimed to explore how future climate change-caused droughts might affect vegetation succession in the region. Plants have two important attributes that regulate water balance: closable pores in leaves (stomata) and long-distance water transport channels (xylem conduits). It was found that the synergistic effect of stomatal adjustment and xylem anti-embolism ability was the key to drought resistance.