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      The Enzymology and Intracellular Organization of Peptide Precursor Processing: The Secretory Vesicle Hypothesis

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          The ‘secretory vesicle hypothesis of precursor processing’ states that the initial endopeptidase cleavages which excise the nascent, biologically active peptides from their protein precursors occur primarily in secretory vesicles (or granules). Hence, all the processing steps subsequent to these cleavages must also occur within these organelles. Two types of evidence are presented in support of this view: (1) cell biological studies which implicate the secretory vesicle as the site of precursor conversion to peptides, and (2) enzymological studies which locate and characterize putative processing enzymes in secretory vesicles. The processing enzymes reviewed include the ‘prohormone-converting enzymes’ which cleave at pairs of basic amino acids, other endopeptidases, carboxypeptidase-B-like enzymes and aminopeptidase, and N-acetylation and α-amidation enzymes. The properties of these enzymes in relation to the nature of the processing micro-environment in the secretory vesicles is discussed.

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          Author and article information

          S. Karger AG
          28 March 2008
          : 40
          : 2
          : 171-184
          Laboratory of Neurochemistry and Neuroimmunology, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Md., USA
          124070 Neuroendocrinology 1985;40:171–184
          © 1985 S. Karger AG, Basel

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          Page count
          Pages: 14
          Progress in Neuroendocrinology


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