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Abstract
<p class="first" id="d3167644e53">Spores of Clostridium aceticum (Wieringa) from 1947
have been revived, and a study
for a redescription of this microorganism has been carried out. C. aceticum was Gram
negative. The cells were rodshaped and peritrichously flagellated. Round spores were
formed in terminal position. The DNA contained 33 mol % guanine plus cytosine. The
organism was obligately anaerobic and grew either chemolithotrophically with H2 +
CO2 or chemoorganotrophically with compounds such as fructose, L-glutamate, L-malate
or pyruvate. H2 and CO2 were converted to acetic acid according to the following equation:
2CO2 + 4H2 leads to CH3COOH + 2H2O The optimal temperature for growth was 30 degrees
C. The optimal pH for chemolithotrophic growth was 8.3. The doubling times for chemolithotrophic
and chemoorganotrophic growth were 25 and 8 h, respectively.
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