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      Antibacterial activities of multi drug resistant Myroides odoratimimus bacteria isolated from adult flesh flies (Diptera: sarcophagidae) are independent of metallo beta-lactamase gene

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          Abstract

          Flesh flies (Diptera: Sarcophagidae) are well known cause of myiasis and their gut bacteria have never been studied for antimicrobial activity against bacteria. Antimicrobial studies of Myroides spp. are restricted to nosocomial strains. A Gram-negative bacterium, Myroides sp., was isolated from the gut of adult flesh flies ( Sarcophaga sp.) and submitted to evaluation of nutritional parameters using Biolog GN, 16S rRNA gene sequencing, susceptibility to various antimicrobials by disc diffusion method and detection of metallo β-lactamase genes (TUS/MUS). The antagonistic effects were tested on Gram-negative and Gram-positive bacteria isolated from human clinical specimens, environmental samples and insect mid gut. Bacterial species included were Aeromonas hydrophila, A. culicicola, Morganella morganii subsp. sibonii, Ochrobactrum anthropi, Weissella confusa, Escherichia coli, Ochrobactrum sp., Serratia sp., Kestersia sp., Ignatzschineria sp., Bacillus sp. The Myroides sp. strain was resistant to penicillin-G, erythromycin, streptomycin, amikacin, kanamycin, gentamycin, ampicillin, trimethoprim and tobramycin. These strain showed antibacterial action against all bacterial strains except W. confusa, Ignatzschineria sp., A. hydrophila and M. morganii subsp. sibonii. The multidrug resistance of the strain was similar to the resistance of clinical isolates, inhibiting growth of bacteria from clinical, environmental and insect gut samples. The metallo β-lactamase (TUS/MUS) genes were absent, and resistance due to these genes was ruled out, indicating involvement of other secretion machinery.

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          Most cited references 39

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          THE EVOLUTION OF STRONG REPRODUCTIVE ISOLATION

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            The neighbour-joining method: a new method for constructing phylogenetic trees

             N Saitou,  M Nei,  J Saitou (1987)
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              Genome sequence of Aeromonas hydrophila ATCC 7966T: jack of all trades.

              The complete genome of Aeromonas hydrophila ATCC 7966(T) was sequenced. Aeromonas, a ubiquitous waterborne bacterium, has been placed by the Environmental Protection Agency on the Contaminant Candidate List because of its potential to cause human disease. The 4.7-Mb genome of this emerging pathogen shows a physiologically adroit organism with broad metabolic capabilities and considerable virulence potential. A large array of virulence genes, including some identified in clinical isolates of Aeromonas spp. or Vibrio spp., may confer upon this organism the ability to infect a wide range of hosts. However, two recognized virulence markers, a type III secretion system and a lateral flagellum, that are reported in other A. hydrophila strains are not identified in the sequenced isolate, ATCC 7966(T). Given the ubiquity and free-living lifestyle of this organism, there is relatively little evidence of fluidity in terms of mobile elements in the genome of this particular strain. Notable aspects of the metabolic repertoire of A. hydrophila include dissimilatory sulfate reduction and resistance mechanisms (such as thiopurine reductase, arsenate reductase, and phosphonate degradation enzymes) against toxic compounds encountered in polluted waters. These enzymes may have bioremediative as well as industrial potential. Thus, the A. hydrophila genome sequence provides valuable insights into its ability to flourish in both aquatic and host environments.
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                Author and article information

                Journal
                Braz J Microbiol
                Braz. J. Microbiol
                bjm
                bjm
                Brazilian Journal of Microbiology
                Sociedade Brasileira de Microbiologia
                1517-8382
                1678-4405
                Apr-Jun 2008
                1 June 2008
                : 39
                : 2
                : 397-404
                Affiliations
                [1 ]Molecular Biology Unit, National Centre for Cell Science, Ganeshkhind , Pune-411 007, Maharashtra, India
                [2 ]Department of Zoology, Modern College of Arts, Science and Commerce, Shivajinagar , Pune-411 005, Maharashtra, India
                [3 ]Present address: Produce and Quality and Safety Laboratory, Henry A. Wallace Beltsville Agricultural Research Center, Agricultural Research Service , 10300 Baltimore Avenue, Beltsville, Maryland 20705, United States
                Author notes
                *Corresponding Author. Mailing address: Molecular Biology Unit, National Centre for Cell Science, Ganeshkhind, Pune-411 007, Maharashtra, India. Tel.: (+91) 20-25708050, Fax: (+91) 20-25692259. E-mail: yogesh@ 123456nccs.res.in
                Article
                S1517-838220080002000035
                10.1590/S1517-838220080002000035
                3768384
                24031236
                © Sociedade Brasileira de Microbiologia

                All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License

                Categories
                Veterinary Microbiology
                Research Paper

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