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      GeneChip analyses of global transcriptional responses of murine macrophages to the lethal toxin of Bacillus anthracis.

      Infection and Immunity
      Animals, Antigens, Bacterial, pharmacology, toxicity, Bacillus anthracis, metabolism, Bacterial Toxins, Gene Expression Profiling, Gene Expression Regulation, Macrophages, immunology, Mice, Mitogen-Activated Protein Kinase Kinases, genetics, Oligonucleotide Array Sequence Analysis, methods, Proteins, Transcription, Genetic

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          Abstract

          We performed GeneChip analyses on RNA from Bacillus anthracis lethal toxin (LeTx)-treated RAW 264.7 murine macrophages to investigate global effects of anthrax toxin on host cell gene expression. Stringent analysis of data revealed that the expression of several mitogen-activated protein kinase kinase-regulatory genes was affected within 1.5 h post-exposure to LeTx. By 3.0 h, the expression of 103 genes was altered, including those involved in intracellular signaling, energy production, and protein metabolism.

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