This paper presents 452 new 21-cm neutral hydrogen line measurements carried out with the FORT receiver of the meridian transit Nan\c{c}ay radiotelescope (NRT) in the period April 2003 -- March 2005. This observational programme is part of a larger project aiming at collecting an exhaustive and magnitude-complete HI extragalactic catalogue for Tully-Fisher applications (the so-called KLUN project, for Kinematics of the Local Universe studies, end in 2008). The whole on-line HI archive of the NRT contains today reduced HI-profiles for ~4500 spiral galaxies of declination delta > -40° (http://klun.obs-nancay.fr). As an example of application, we use direct Tully-Fisher relation in three (JHK) bands in deriving distances to a large catalog of 3126 spiral galaxies distributed through the whole sky and sampling well the radial velocity range between 0 and 8000 km/s. Thanks to an iterative method accounting for selection bias and smoothing effects, we show as a preliminary output a detailed and original map of the velocity field in the Local Universe.