In this paper we applied for the first time the no-signaling in time ( NSIT) formalism discussed by Kofler and Brukner (2013) to investigate temporal entanglement between binary human behavioral unconscious choices at t1 with binary random outcomes at t2. NSIT consists of a set of inequalities and represents mathematical conditions for macro-realism which require only two measurements in time. The analyses of three independent experiments show a strong violation of NSIT in two out of three of them, suggesting the hypothesis of a quantum-like temporal entanglement between human choices at t1 with binary random outcomes at t2. We discuss the potentialities of using NSIT to test temporal entanglement with behavioral measures.