16
views
0
recommends
+1 Recommend
1 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: found
      Is Open Access

      Conjunctival microbial flora of clinically normal persons who work in a hospital environment Translated title: Microbiota da conjuntiva de indivíduos clinicamente normais que trabalham em ambiente hospitalar

      research-article

      Read this article at

      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          The objective of the work was to study the microbiota of the conjunctival secretion of health professionals. Samples were collected from the clinically normal eyes of 40 health professionals in four different sectors, Proctology, General Intensive Care Unit (ICU), Male Ward, and Oncology of Hospital das Clínicas Dr. Augusto Leite, Aracaju, SE, Brazil. Ten professionals from each sector were selected. The samples were inoculated into various culture media: blood agar, Chapman agar, EMB medium (Teague), and Sabouraud dextrose agar with chloramphenicol. The bacteria most frequently isolated from all the sectors were Staphylococcus epidermidis (45.0%) and Bacillus sp (29.0%). The least frequent bacteria were Proteus sp (6.1%), Staphylococcus aureus (4.1%), Enterobacter sp (4.1%), Alcaligenes sp (4.1%), Citrobacter sp (2.1%), Moraxella sp (2.1%), and Proteus mirabilis (2.1%). Fungi were not isolated. These results confirm the continuous contamination of the conjunctival sac of these professionals by the external environment, while at the same time confirming that the mechanisms of local defence continue to be intact, preventing the fixation of invading microorganisms.

          Translated abstract

          O objetivo deste trabalho foi a caracterização da microbiota da secreção conjuntival de profissionais hígidos do Hospital da Clínicas Dr. Augusto Leite, Aracaju, SE, Brasil. Foram colhidas amostras de secreção conjuntival de olhos clinicamente normais de 40 profissionais em 4 diferentes setores do hospital: Proctologia, Unidade Intensiva de Tratamento Geral, Enfermaria Masculina e Setor de Oncologia. As amostras foram inoculadas em ágar sangue, ágar Chapman, meio de Teague e ágar Sabouraud dextrose com cloranfenicol. As bactérias mais frequentemente isoladas de todos os setores foram Staphylococcus epidermidis (45,0%) e Bacillus sp (29,0%). As menos frequentes foram Proteus sp (6,1%), Staphylococcus aureus (4,1%), Enterobacter sp (4,1%), Alcaligenes sp (4,1%), Citrobacter sp (2,1%), Moraxella sp (2,1%) e Proteus mirabilis (2,1%). Fungos não foram isolados. Estes resultados confirmam uma contaminação contínua do saco conjuntival desses profissionais pelo ambiente externo, evidenciando a importância dos mecanismos locais de defesa, que devem estar intactos na prevenção contra a fixação e a invasão desses microrganismos.

          Related collections

          Most cited references23

          • Record: found
          • Abstract: found
          • Article: not found

          Quantitative alterations of the commensal eye bacteria in contact lens wear.

          A study was performed on the commensal external eye flora in 34 long-term contact lens wearers and a matched control group, in order to identify the effect of lens wear. Samples were taken from the lid margin and conjunctiva by semi-quantitative, and tear film by quantitative methods. Cultured bacteria were identified with particular attention to coagulase-negative staphylococci. No qualitative alteration in the commensal bacteria was found, although lens wearers were found to have a significantly higher number of species at all sites than controls. Significant quantitative changes were identified on the lid margin, with particularly high counts in some lens wearers, and tear film. Increased numbers of bacteria obtained from the conjunctiva were not statistically significant. Quantitative changes in the tear film are thought to be secondary to changes at the lid margin, for which no explanation is apparent.
            Bookmark
            • Record: found
            • Abstract: found
            • Article: not found

            Potential sources of bacteria that are isolated from contact lenses during wear.

            The aim of this paper was to determine the possible contamination sources of contact lenses during wear. Potential sources of the microbiota that colonized hydrogel contact lenses during wear were examined. The microorganisms that colonize contact lenses were grown, identified, and compared to those microorganisms that colonized the lower lid margins, upper bulbar conjunctiva, hands, and contact lens cases of contact lens wearers. In addition, the incidence of contamination of the domestic water supply in the Sydney area was obtained, and this was compared to the incidence of colonization of contact lenses by microorganisms in general and gram-negative bacteria in particular. There was a wide diversity of bacteria that were isolated from each site sampled. Coagulase-negative staphylococci and Propionibacterium spp. were the most common isolates from all ocular sites examined, and constituted the normal ocular microbiota. Other bacteria, including members of the families Enterobacteriaceae and Pseudomonadaceae, were isolated infrequently from all sites, but most frequently from contact lens cases. Statistical analysis revealed that there was a correlation between the isolation of bacteria from the contact lens and the lower lid margin (p < 0.001). Analysis of this correlation revealed that this was true for the normal microbiota. A correlation was also noted between the colonization of contact lenses by gram-negative bacteria and contamination of the domestic water supply. This study has demonstrated that the likely route for the normal ocular microbiota colonizing contact lenses is via the lid margins, whereas colonization by gram-negative bacteria, including potential agents of microbial keratitis, is likely to be from the domestic water supply.
              Bookmark
              • Record: found
              • Abstract: not found
              • Article: not found

              Anatomia Fundamental

                Bookmark

                Author and article information

                Contributors
                Role: ND
                Role: ND
                Role: ND
                Journal
                bjm
                Brazilian Journal of Microbiology
                Braz. J. Microbiol.
                Sociedade Brasileira de Microbiologia (São Paulo )
                1678-4405
                March 2000
                : 31
                : 1
                : 12-16
                Affiliations
                [1 ] Universidade Federal de Sergipe Brazil
                [2 ] Universidade Federal de Minas Gerais Brazil
                Article
                S1517-83822000000100004
                10.1590/S1517-83822000000100004
                c55cf7ff-ecfa-4751-b625-4ad018530442

                http://creativecommons.org/licenses/by/4.0/

                History
                Product

                SciELO Brazil

                Self URI (journal page): http://www.scielo.br/scielo.php?script=sci_serial&pid=1517-8382&lng=en
                Categories
                MICROBIOLOGY

                Microbiology & Virology
                conjunctival microbiota,hospital environment,Staphylococcus epidermidis,Staphylococcus aureus,microbiota conjuntival,ambiente hospitalar

                Comments

                Comment on this article