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      Engineering Escherichia coli coculture systems for the production of biochemical products.

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          Abstract

          Engineering microbial consortia to express complex biosynthetic pathways efficiently for the production of valuable compounds is a promising approach for metabolic engineering and synthetic biology. Here, we report the design, optimization, and scale-up of an Escherichia coli-E. coli coculture that successfully overcomes fundamental microbial production limitations, such as high-level intermediate secretion and low-efficiency sugar mixture utilization. For the production of the important chemical cis,cis-muconic acid, we show that the coculture approach achieves a production yield of 0.35 g/g from a glucose/xylose mixture, which is significantly higher than reported in previous reports. By efficiently producing another compound, 4-hydroxybenzoic acid, we also demonstrate that the approach is generally applicable for biosynthesis of other important industrial products.

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          Author and article information

          Journal
          Proc. Natl. Acad. Sci. U.S.A.
          Proceedings of the National Academy of Sciences of the United States of America
          1091-6490
          0027-8424
          Jul 7 2015
          : 112
          : 27
          Affiliations
          [1 ] Chemical Engineering Department, Massachusetts Institute of Technology, Cambridge, MA 02139.
          [2 ] Chemical Engineering Department, Massachusetts Institute of Technology, Cambridge, MA 02139 gregstep@mit.edu.
          Article
          1506781112
          10.1073/pnas.1506781112
          26111796
          c577e610-7324-4772-860b-6775df8e6c83
          History

          4-hydroxybenzoic acid,metabolic engineering,microbial coculture,muconic acid

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