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      Nuclear pore complex is able to transport macromolecules with diameters of about 39 nm.

      1 ,

      Molecular biology of the cell

      American Society for Cell Biology (ASCB)

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          Abstract

          Bidirectional transport of macromolecules between the nucleus and the cytoplasm occurs through the nuclear pore complexes (NPCs) by a signal-mediated mechanism that is directed by targeting signals (NLSs) residing on the transported molecules or "cargoes." Nuclear transport starts after interaction of the targeting signal with soluble cellular receptors. After the formation of the cargo-receptor complex in the cytosol, this complex crosses the NPC. Herein, we use gold particles of various sizes coated with cargo-receptor complexes to determine precisely how large macromolecules crossing the NPC by the signal-mediated transport mechanism could be. We found that cargo-receptor-gold complexes with diameter close to 39 nm could be translocated by the NPC. This implies that macromolecules much larger than the assumed functional NPC diameter of 26 nm can be transported into the karyoplasm. The physiological relevance of this finding was supported by the observation that intact nucleocapsids of human hepatitis B virus with diameters of 32 and 36 nm are able to cross the nuclear pore without disassembly.

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          Author and article information

          Journal
          Mol. Biol. Cell
          Molecular biology of the cell
          American Society for Cell Biology (ASCB)
          1059-1524
          1059-1524
          Feb 2002
          : 13
          : 2
          Affiliations
          [1 ] Swiss Federal Institute of Technology Zurich, Institute of Biochemistry, CH-8092 Zurich, Switzerland. pante@zoology.ubc.ca
          Article
          10.1091/mbc.01-06-0308
          65638
          11854401

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