6
views
0
recommends
+1 Recommend
1 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: not found

      Evaluation of the antimicrobial activity of the mastoparan Polybia-MPII isolated from venom of the social wasp Pseudopolybia vespiceps testacea (Vespidae, Hymenoptera).

      Read this article at

      ScienceOpenPublisherPubMed
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Mastoparans, a class of peptides found in wasp venom, have significant effects following a sting as well as useful applications in clinical practice. Among these is their potential use in the control of micro-organisms that cause infectious diseases with a significant impact on society. Thus, the present study describes the isolation and identification of a mastoparan peptide from the venom of the social wasp Pseudopolybia vespiceps and evaluated its antimicrobial profile against bacteria (Staphylococcus aureus and Mycobacterium abscessus subsp. massiliense), fungi (Candida albicans and Cryptococcus neoformans) and in vivo S. aureus infection. The membrane pore-forming ability was also assessed. The mastoparan reduced in vitro and ex vivo mycobacterial growth by 80% at 12.5 µM in infected peritoneal macrophages but did not affect the shape of bacterial cells at the dose tested (6.25 µM). The peptide also showed potent action against S. aureus in vitro (EC50 and EC90 values of 1.83 µM and 2.90 µM, respectively) and reduced the in vivo bacterial load after 6 days of topical treatment (5 mg/kg). Antifungal activity was significant, with EC50 and EC90 values of 12.9 µM and 15.3 µM, respectively, for C. albicans, and 11 µM and 22.70 µM, respectively, for C. neoformans. Peptides are currently attracting interest for their potential in the design of antimicrobial drugs, particularly due to the difficulty of micro-organisms in developing resistance to them. In this respect, Polybia-MPII proved to be highly effective, with a lower haemolysis rate compared with peptides of the same family.

          Related collections

          Author and article information

          Journal
          Int. J. Antimicrob. Agents
          International journal of antimicrobial agents
          Elsevier BV
          1872-7913
          0924-8579
          Feb 2017
          : 49
          : 2
          Affiliations
          [1 ] Department of Physiological Sciences, Institute of Biological Sciences, University of Brasília, Brasília, Brazil.
          [2 ] Department of Microbiology, Immunology, Parasitology and Pathology, Institute of Tropical Diseases and Public Heath, Federal University of Goiás, Goiânia, Brazil.
          [3 ] Department of Cell Biology, Institute of Biological Sciences, University of Brasília, Brasília, Brazil.
          [4 ] Center for Proteomics and Biochemical Analyses, Genomic Science and Biotechnology Graduate Program, Catholic University of Brasília, Brasília, Brazil; Molecular Pathology Graduate Program, University of Brasilia, Brasília, Brazil.
          [5 ] Center for Proteomics and Biochemical Analyses, Genomic Science and Biotechnology Graduate Program, Catholic University of Brasília, Brasília, Brazil; Department of Biology, Institute of Biological Sciences, Federal University of Juiz de Fora, Juiz de Fora, Brazil.
          [6 ] Laboratory of Immunopathology, Butantan Institute, Sao Paulo, SP, Brazil.
          [7 ] Department of Physiological Sciences, Institute of Biological Sciences, University of Brasília, Brasília, Brazil; Laboratory of Mass Spectrometry, Embrapa Genetic Resources and Biotechnology, Brasília, Brazil.
          [8 ] Department of Physiological Sciences, Institute of Biological Sciences, University of Brasília, Brasília, Brazil; Laboratory of Immunopathology, Butantan Institute, Sao Paulo, SP, Brazil.
          [9 ] Center for Proteomics and Biochemical Analyses, Genomic Science and Biotechnology Graduate Program, Catholic University of Brasília, Brasília, Brazil; Molecular Pathology Graduate Program, University of Brasilia, Brasília, Brazil; Department of Biology, Institute of Biological Sciences, Federal University of Juiz de Fora, Juiz de Fora, Brazil; S-Inova Biotech, Graduate Biotechnology Program, Dom Bosco Catholic University, Campo Grande, Brazil.
          [10 ] Department of Physiological Sciences, Institute of Biological Sciences, University of Brasília, Brasília, Brazil. Electronic address: mmortari@unb.br.
          Article
          S0924-8579(17)30006-7
          10.1016/j.ijantimicag.2016.11.013
          28108242

          Comments

          Comment on this article