This investigation was performed to determine quantitatively the ranges of ablation and to evaluate the morphological changes in human enamel and dentin irradiated by Er,Cr:YSGG laser with or without water spray. Recently, Er,Cr:YSGG laser has been introduced in dental clinics to remove carious dental hard tissues in anticipation of replacing the high-speed dental drill. A total of 40 extracted human teeth were used in this study. An Er,Cr:YSGG laser was used to ablate human dental hard tissues with the output powers of 3 to 6 W. Ablation extent with or without water spray at different output powers was measured, and the morphological changes on enamel and dentin were also investigated by stereoscopy and scanning electron microscopy (SEM). The irradiation with water spray significantly (p < 0.001) increased the ablation depths compared to those irradiated without water mist. Morphological findings by SEM indicated that when irradiated without water spray, carbonization with brown or dark color was recognized in enamel or dentin, respectively. In addition, cavities with a molten lava-like appearance were produced and an irregular structure with many microholes was observed in dentin. These results suggest that during the Er,Cr:YSGG laser irradiation, water spray directed at the ablation sites increases the ablation depths and water plays an important role as an initiator of the ablation of dental hard tissues.