In vitro Production of ‘Glomerular Red Cells’: Role of pH and Osmolality

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Abstract

We used an in vitro model consisting of a polycarbonate filter with pore diameters of 3 μm, through which red cells suspended in urine or buffer were pumped at a flow rate of 0.5 ml/min. pH and osmolality of the suspension were systematically changed within a range of pH 5–8 and 200–800 mosm/kg H<sub>2</sub>O. It was found that: (1) glomerular shapes could be produced by this filtration technique; (2) urine facilitated the production of glomerular shapes; (3) certain shapes occurred predominantly, at specific conditions of pH and osmolality and (4) hemolysis decreased with increasing osmolality or very low pH. These observations shed light on the mechanism by which glomerular red cell shapes are formed.

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Journal

Journal ID (publisher-id): NEF

Journal ID (nlm-ta): Nephron

Journal ID (doi): 10.1159/issn.1660-8151

Title: Nephron

Publisher: S. Karger AG

ISSN (Print): 1660-8151

ISSN (Electronic): 2235-3186

Publication date Subscription-year: 1990

Publication date (Print): 1990

Publication date (Electronic): 10 December 2008

Volume: 56

Issue: 1

Pages: 13-18

Affiliations

Department of Internal Medicine, University of Berne, Inselspital Berne, Switzerland

Article

Publisher ID: 186093 Other ID: Nephron 1990;56:13–18

DOI: 10.1159/000186093

PubMed ID: 2234244

SO-VID: c5cb1fe6-7da2-4b83-944e-28dc3ccf5942

License:

Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

History

Date accepted : 21 November 1989

Page count

Pages: 6

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