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      Bacteriostatic effect of lipopeptides from Bacillus subtilis N-2 on Pseudomonas putida using soybean meal by solid-state fermentation

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          Abstract

          Bacillus subtilis N-2 which was isolated from natto, produced lipopeptides using soybean meal as a substrate. This work aimed to purify, identify, and determine the antibacterial mechanism of lipopeptides produced by B. subtilis N-2. The fermented product obtained by solid-state fermentation was subjected to water extraction, acid precipitation, and methanol extraction. Fractions were separated and collected using a two-step ultrafiltration method and then identified by LC–MS/MS. Mass spectrometry characterization revealed the presence of four variants of iturin A that differed according to the β-amino fatty acid chain from C 14 to C 17 as well as the amino acid positions. A new lipopeptide (m/z 1070.3) was identified and its structure was different from the previously reported lipopeptides. The lipopeptides were shown to inhibit the growth of an isolate of Pseudomonas putida, a common pathogen in decaying fish, by changing membrane permeability. These results suggest that the lipopeptides from B. subtilis N-2 could be used as a biocontrol agent in aquaculture.

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          Author and article information

          Journal
          MLST
          Marine Life Science & Technology
          Springer (China )
          2096-6490
          2662-1746
          01 May 2020
          25 February 2020
          : 2
          : 2
          : 172-180
          Affiliations
          1School of Food Science and Engineering, Ocean University of China, Yushan Road 5, Qingdao 266003, Shandong, China
          Author notes
          *Corresponding authors: Haijin Mou, E-mail: mousun@ 123456ouc.edu.cn ; Qing Kong, E-mail: kongqing@ 123456ouc.edu.cn
          Article
          s42995-020-00028-0
          10.1007/s42995-020-00028-0
          © 2020 Ocean University of China
          Product
          Self URI (journal-page): https://www.springer.com/journal/42995
          Categories
          Research Paper

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