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      An extremely sensitive aptasensor based on interfacial energy transfer between QDS SAMs and GO.

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          Abstract

          In this work we designed a fluorescent self-assemblied multilayers, with thrombin aptamers and ssDNA as aptamer fixed onto the outermost layer, respectively. This multilayers can effectively sense biomolecules by interfacial florescence resonance energy transfer from multilayers to graphene oxide. High fluorescence quenching efficiency of graphene oxide and self-assemblied membrane' concentration results in good sensitivity for biosensing. A new interfacial sensing method with extremely high sensitivity for thrombin and DNA sequence was established, and the detection limit for thrombin and DNA was 16.2pM and 72.6 fM, respectively.

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          Author and article information

          Journal
          Spectrochim Acta A Mol Biomol Spectrosc
          Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy
          1873-3557
          1386-1425
          Oct 15 2014
          : 131
          Affiliations
          [1 ] College of Materials Science and Engineering, Huaqiao University, 361021 Xiamen, China. Electronic address: sunxy@hqu.edu.cn.
          [2 ] College of Materials Science and Engineering, Huaqiao University, 361021 Xiamen, China.
          Article
          S1386-1425(14)00666-0
          10.1016/j.saa.2014.04.093
          24835931
          Copyright © 2014 Elsevier B.V. All rights reserved.

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