Hepatoprotective potential of Fagonia olivieri DC. against acetaminophen induced toxicity in rat

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Abstract

Background

Fagonia olivieri (DC) being used for the treatment of diabetes, cancer, fever and claimed to be effective in many other stress related disorders. In this study we have evaluated the F. olivieri whole methanol extract and its derived fractions for various in vitro and in vivo antioxidant studies.

Methods

The crude methanol extract of the whole plant of F. olivieri (FOM) and its derived fractions; n-hexane (FOH), chloroform (FOC), ethyl acetate (FOE), n-butanol (FOB) and aqueous (FOA) were evaluated for the total phenolic and flavonoid content and in vitro antioxidant abilities. The antioxidant effect of FOM was determined by acetaminophen-induced hepatotoxicity in Sprague–Dawley ( Rattus novergicus) male rats. The methanol/fractions were also analysed by HPLC analysis for the presence of polyphenolics.

Results

The total phenolic content of the samples ranged from 19.3 ± 0.529 to 106.2 ± 0.892 mg GAE/g extract while total flavonoid content 16.2 ± 0.881 to 50.1 ± 1.764 mg RTE/g extract, respectively. FOA showed highest radical scavenging activity for DPPH (IC ₅₀ = 55.2 ± 1.212 μg/ml), ABTS (IC ₅₀ = 90.2 ± 1.232 μg/ml) superoxide (IC ₅₀ = 37.1 ± 0.643 μg/ml) and for H ₂O ₂ (IC ₅₀ = 64 ± 1.463 μg/ml). FOE exhibited the highest antioxidant activities for phosphomolybdenum (IC ₅₀ = 78.2 ± 0.883 μg/ml) and for hydroxyl radical scavenging (IC ₅₀ = 82 ± 2.603 μg/ml). HPLC analysis of FOM and its derived fractions showed the presence of rutin, catechin and gallic acid. Elevated levels of AST, ALT, ALP, LDH and lipid profile in serum and lipid peroxidation and DNA damages in liver; while decreased activity level of CAT, SOD, GSH-Px, GR and reduced glutathione (GSH) concentration induced with acetaminophen in rat were reverted towards the control group with co-administration of FOM.

Conclusion

Our results showed that F. olivieri is a potential source of natural antioxidants, which justifies its use in folklore medicine.

Related collections

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Author and article information

Contributors

Umbreen Rashid: umbreen.rashid@gmail.com

Muhammad Rashid Khan: mrkhanqau@yahoo.com

Moniba Sajid: moniba_qau@yahoo.com

Journal

Journal ID (nlm-ta): BMC Complement Altern Med

Journal ID (iso-abbrev): BMC Complement Altern Med

Title: BMC Complementary and Alternative Medicine

Publisher: BioMed Central (London )

ISSN (Electronic): 1472-6882

Publication date (Electronic): 9 November 2016

Publication date PMC-release: 9 November 2016

Publication date Collection: 2016

Volume: 16

Electronic Location Identifier: 449

Affiliations

[1 ]Department of Environmental Sciences, GC Women University Sialkot, Sialkot, Pakistan

[2 ]Department of Biochemistry, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, 45320 Pakistan

Article

Publisher ID: 1445

DOI: 10.1186/s12906-016-1445-x

PMC ID: 5103455

PubMed ID: 27829418

SO-VID: c6310604-fc1c-49df-b782-aced8434794e

License:

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License ( http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver ( http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.