The fungal airway microbiome in cystic fibrosis and non-cystic fibrosis bronchiectasis

Highlights

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  The prevalence of fungal disease is increasing in CF and non-CF bronchiectasis.

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  Effective management of fungal disease requires an understanding of the mycobiome.

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  Culture methods alone are inadequate for the accurate diagnosis of fungal disease.

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  Our study provides a framework to characterize fungal airway disease using NGS.

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  NGS can improve detection and clinical management of fungal infections.

Abstract

Background

The prevalence of fungal disease in cystic fibrosis (CF) and non-CF bronchiectasis is increasing and the clinical spectrum is widening. Poor sensitivity and a lack of standard diagnostic criteria renders interpretation of culture results challenging. In order to develop effective management strategies, a more accurate and comprehensive understanding of the airways fungal microbiome is required. The study aimed to use DNA sequences from sputum to assess the load and diversity of fungi in adults with CF and non-CF bronchiectasis.

Methods

Next generation sequencing of the ITS2 region was used to examine fungal community composition (n = 176) by disease and underlying clinical subgroups including allergic bronchopulmonary aspergillosis, chronic necrotizing pulmonary aspergillosis, non-tuberculous mycobacteria, and fungal bronchitis. Patients with no known active fungal disease were included as disease controls.

Results

ITS2 sequencing greatly increased the detection of fungi from sputum. In patients with CF fungal diversity was lower, while burden was higher than those with non-CF bronchiectasis. The most common operational taxonomic unit (OTU) in patients with CF was Candida parapsilosis (20.4%), whereas in non-CF bronchiectasis sputum Candida albicans (21.8%) was most common. CF patients with overt fungal bronchitis were dominated by Aspergillus spp. , Exophiala spp., Candida parapsilosis or Scedosporium spp.

Conclusion

This study provides a framework to more accurately characterize the extended spectrum of fungal airways diseases in adult suppurative lung diseases.