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      Ghrelin and des-acyl ghrelin: two major forms of rat ghrelin peptide in gastrointestinal tissue.

      Biochemical and Biophysical Research Communications

      Amino Acid Sequence, Animals, Duodenum, metabolism, Ghrelin, Immunoglobulins, immunology, Male, Molecular Sequence Data, Peptide Hormones, Peptides, analysis, Radioimmunoassay, Rats, Rats, Sprague-Dawley, Stomach, Tissue Distribution

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          Abstract

          Ghrelin, a novel peptide purified from stomach, is the endogenous ligand for the growth hormone secretagogue receptor and has potent growth hormone-releasing activity. The Ser3 residue of ghrelin is modified by n-octanoic acid, a modification necessary for hormonal activity. We established two ghrelin-specific radioimmunoassays; one recognizes the octanoyl-modified portion and another the C-terminal portion of ghrelin. Using these radioimmunoassay systems, we found that two major molecular forms exist-ghrelin and des-n-octanoyl ghrelin. While ghrelin activates growth-hormone secretagogue (GHS) receptor-expressing cells, the nonmodified des-n-octanyl form of ghrelin, designated as des-acyl ghrelin, does not. In addition to these findings, our radioimmunoassay systems also revealed high concentrations of ghrelin in the stomach and small intestine.

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          Journal
          11162448
          10.1006/bbrc.2000.4039

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