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      Conservation of miR-15a/16-1 and miR-15b/16-2 clusters.

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          Abstract

          MiR-15a/16-1 and miR-15b/16-2 clusters have been shown to play very important roles in regulating cell proliferation and apoptosis by targeting cell cycle proteins and the antiapoptotic Bcl-2 gene. However, the physiological implications of those two clusters are largely elusive. By aligning the primary miR-15a/16-1 sequence among 44 vertebrates, we found that there was a gap in the homologous region of the rat genome. To verify that there was a similar miR-15a/16-1 cluster in rats, we amplified this region from rat genomic DNA using PCR and found that a 697-bp sequence was missing in the current rat genome database, which covers the miR-15a/16-1 cluster. Subsequently, we also investigated the expression pattern of individual miRNAs spliced from miR-15a/16-1 and miR-15b/16-2 clusters, including miR-15a, miR-15a*, miR-15b, miR-15b*, miR-16-1/2, and miR-16-1/2* from various rat tissues, and found that all of those miRNAs were expressed in the investigated tissues. MiR-16 was most expressed in the heart, followed by the brain, lung, kidney, and small intestine, which indicates tissue specificity for individual miRNA expression from both clusters. Our results demonstrated that both miR-15a/16-1 and miR-15b/16-2 clusters are highly conserved among mammalian species. The investigation of the biological functions of those two clusters using transgenic or knockout/knockdown models will provide new clues to understanding their implications in human diseases and finding a new approach for miRNA-based therapy.

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          Author and article information

          Journal
          Mamm Genome
          Mammalian genome : official journal of the International Mammalian Genome Society
          Springer Science and Business Media LLC
          1432-1777
          0938-8990
          Feb 2010
          : 21
          : 1-2
          Affiliations
          [1 ] Department of Physiology, University of Tennessee Health Science Center, CRB, 19 S. Manassas St, Memphis, TN, 38163, USA. jyue@utmem.edu
          Article
          NIHMS172083
          10.1007/s00335-009-9240-3
          2820079
          20013340
          c69dc14c-92c0-4b2f-9325-13139ae97d48
          History

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