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      Regulation of cytosolic free Ca2+ in cultured rat alveolar macrophages (NR8383).

      Journal of Leukocyte Biology
      3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester, pharmacology, Adenosine Triphosphate, Animals, Calcium, metabolism, Calcium-Transporting ATPases, antagonists & inhibitors, Cells, Cultured, Cytosol, Enzyme Inhibitors, Imidazoles, Inositol 1,4,5-Trisphosphate, biosynthesis, Macrophages, Alveolar, Membrane Potentials, Rats, Receptors, Purinergic P2, physiology, Thapsigargin, Zymosan

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          Abstract

          Ca2+ mobilization in the rat alveolar macrophage cell line NR8383 was examined with the Ca2+-sensitive fluorescent probe Fura-2. ATP and norepinephrine elicited a 108 and 46% increase, respectively, in cytosolic free Ca2+ concentration ([Ca2+]i). Acetylcholine, nicotine, isoproterenol, substance P, and vasoactive intestinal polypeptide did not alter [Ca2+]i. Inositol 1,4,5-trisphosphate (IP3) formation was also activated by ATP. The carbohydrate-rich cell wall preparation, zymosan, induced a gradual [Ca2+]i, increase only in the presence of external Ca2+, but did not activate IP3 formation. This increase was abolished by laminarin and by removal of extracellular Ca2+, suggesting that the [Ca2+]i increase was activated by beta-glucan receptors and mediated by Ca2+ influx. This influx was significantly reduced by SKF96365, but not by nifedipine, (omega-conotoxin GVIA, (omega-agatoxin IVA, or flunarizine. These results suggest that release of intracellular Ca2+ in NR8383 cells is regulated by P2-purinoceptors and that zymosan causes Ca2+ influx via a receptor-operated pathway.

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