Development and validation of forensically useful growth models for Central European population of Creophilus maxillosus L. (Coleoptera: Staphylinidae)

Abstract

The hairy rove beetle, Creophilus maxillosus (Linnaeus) (Staphylinidae), is recognized for its use in forensic entomology. However, insufficient developmental data exist for the Central European population of this species. Accordingly, we studied the development of C. maxillosus at ten constant temperatures (10–32.5 °C). Based on these results, linear and nonlinear developmental models were created and validated . We also studied the effect of different homogenous diets (third-instar larvae or puparia of Calliphora sp. Robineau-Desvoidy or Lucilia sp. Robineau-Desvoidy (Diptera: Calliphoridae) or mix of first- and second-instar larvae of Necrodes littoralis (Linnaeus) (Coleoptera: Silphidae)) on the development and mortality of C. maxillosus. Average total development times ranged between 122.21 days at 15 °C and 22.18 days at 30 °C. Beetles reached the adult stage in seven out of ten temperatures (15–30 °C). No beetles reached the adult stage when fed with larvae of N. littoralis; their development times at first and second larval stage were also significantly longer than in other food conditions . When C. maxillosus larvae were fed with blowfly larvae, the highest mortality was observed at the pupal stage, as compared when they were fed with blowfly puparia—at the first larval stage . While validating thermal summation models, the highest age estimation errors were found for beetles bred at 10 and 12.5 °C (between 21 and 43% for all developmental events). Age estimation errors were on average higher for pupation and eclosion than hatching and first and second ecdyses. While validating the models with specimens fed with different diets, the highest errors were recorded for beetles fed with N. littoralis larvae (22% for the first ecdysis and 33% for the second ecdysis) and Lucilia sp. puparia (32% for pupation and 22% for eclosion). Implications for C. maxillosus use in forensic entomology are discussed.

Key Points • Development of the Central European population of C. maxillosus was studied at ten constant temperatures and using different homogenous diets. • Thermal summation models were validated with insects reared at different temperatures and fed with different diets. • Total development times ranged between 122 days at 15 °C and 22 days at 30 °C. Beetles reached the adult stage in seven temperatures (15–30 °C). • The highest age estimation errors were found for beetles bred at 10 and 12.5 °C (21–43%) and for beetles fed with Necrodes littoralis larvae (22–33%). • The lowest mortality was observed for beetles fed with Calliphora sp. and Lucilia sp. larvae. Estimation errors were generally low for beetles fed with blowfly larvae or Calliphora sp. puparia.

Electronic supplementary material

The online version of this article (10.1007/s00414-020-02275-3) contains supplementary material, which is available to authorized users.