22
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: not found

      Pseudomonas fluorescens Pf-5 genome-wide mutant screen for resistance to the antimicrobial peptide alfalfa snakin-1.

      Read this article at

      ScienceOpenPublisherPubMed
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Snakin-1, a peptide produced by higher plants, has broad-spectrum antibiotic activity, inhibiting organisms ranging from Bacteria to Eukaryotes. However, the mode of action against target organisms is poorly understood. As a first step to elucidate the mechanism, we screened a mutation library of Pseudomonas fluorescens Pf-5 in LB and agar medium supplemented with alfalfa snakin-1 (MsSN1). We identified three biofilm formation-related Pseudomonas mutants that showed increased resistance to MsSN1. Genetic, physiological and bioinformatics analysis validated the results of the mutant screens, indicating that bacterial adhesion protein lapA is probably the target of MsSN1. Collectively, these findings suggest that snakin-1 acts on microbial adhesion properties.

          Related collections

          Author and article information

          Journal
          FEMS Microbiol. Lett.
          FEMS microbiology letters
          1574-6968
          0378-1097
          Jan 2015
          : 362
          : 2
          Affiliations
          [1 ] Instituto de Genética Ewald A. Favret (CICVyA-INTA), De los reseros S/N, Castelar C25 (1712), Buenos Aires, Argentina Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Avda. Rivadavia 1917, C1033AAJ, Cuidad Autónoma de Buenos Aires, Argentina.
          [2 ] Instituto de Genética Ewald A. Favret (CICVyA-INTA), De los reseros S/N, Castelar C25 (1712), Buenos Aires, Argentina.
          [3 ] School of Biosciences and Biotechnology, University of Camerino, 62032 Camerino (MC), Italy.
          [4 ] Instituto de Genética Ewald A. Favret (CICVyA-INTA), De los reseros S/N, Castelar C25 (1712), Buenos Aires, Argentina Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Avda. Rivadavia 1917, C1033AAJ, Cuidad Autónoma de Buenos Aires, Argentina gabysoto80@gmail.com.
          Article
          fnu006
          10.1093/femsle/fnu006
          25670697
          c7cb2402-bcce-4087-9f00-248ba8f1d29e
          © FEMS 2015. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.
          History

          action mechanism,antimicrobial activity,antimicrobial peptides,innate immunity

          Comments

          Comment on this article