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      Immunohistochemical Study of a Tubular Basement Membrane Antigen in Normal Human Urinary Sediment by a Monoclonal Antibody

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          Abstract

          This preliminary study concerns murine monoclonal antibodies (Mabs) against tubular basement membranes (TBM) of human kidney which were produced by the classical hybridization technique. One of these Mabs (PP8–1) specifically recognized an antigen present in the tubular basement membranes of various portions of tubule and in the Bowman’s capsule. By immunocytochemical techniques, this Mab was used to study the presence and the distribution of the related antigen in urinary sediment from 12 normal healthy subjects. This TBM and Bowman’s capsule antigen was demonstrated along hyaline urinary cast surface and, in some cases, in free extracellular fibrillar structures morphologically unrelated to the casts. This suggests that, in some normal conditions, catabolism of TBM and/or Bowman’s capsule could release material in urine, as it has been described for glomerular basement membrane (GBM) constituents. Such an immunocytochemical approach could be useful to detect components from tubular renal basement membranes in urinary sediment during pathological conditions.

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          Author and article information

          Journal
          NEF
          Nephron
          10.1159/issn.1660-8151
          Nephron
          S. Karger AG
          1660-8151
          2235-3186
          1989
          1989
          10 December 2008
          : 53
          : 4
          : 343-348
          Affiliations
          INSERM U133, Laboratoire d’Explorations Biologiques Cellulaires, CHU Purpan et Laboratoire d’Optique Electronique du CNRS, Toulouse Rangueil, France
          Article
          185779 Nephron 1989;53:343–348
          10.1159/000185779
          2689899
          © 1989 S. Karger AG, Basel

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          Page count
          Pages: 6
          Categories
          Original Paper

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