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      Live imaging of astrocyte responses to acute injury reveals selective juxtavascular proliferation.

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          Abstract

          Astrocytes are thought to have important roles after brain injury, but their behavior has largely been inferred from postmortem analysis. To examine the mechanisms that recruit astrocytes to sites of injury, we used in vivo two-photon laser-scanning microscopy to follow the response of GFP-labeled astrocytes in the adult mouse cerebral cortex over several weeks after acute injury. Live imaging revealed a marked heterogeneity in the reaction of individual astrocytes, with one subset retaining their initial morphology, another directing their processes toward the lesion, and a distinct subset located at juxtavascular sites proliferating. Although no astrocytes actively migrated toward the injury site, selective proliferation of juxtavascular astrocytes was observed after the introduction of a lesion and was still the case, even though the extent was reduced, after astrocyte-specific deletion of the RhoGTPase Cdc42. Thus, astrocyte recruitment after injury relies solely on proliferation in a specific niche.

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          Author and article information

          Journal
          Nat Neurosci
          Nature neuroscience
          Springer Science and Business Media LLC
          1546-1726
          1097-6256
          May 2013
          : 16
          : 5
          Affiliations
          [1 ] Physiological Genomics, Institute of Physiology, Ludwig-Maximilians University Munich, Munich, Germany.
          Article
          nn.3371
          10.1038/nn.3371
          23542688
          c7e21353-469b-4ac3-b6bf-73fed7fcc18a
          History

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