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ADP ribosylation factor 1 activity is required to recruit AP-1 to the major histocompatibility complex class I (MHC-I) cytoplasmic tail and disrupt MHC-I trafficking in HIV-1-infected primary T cells.

Journal of Biology

ADP-Ribosylation Factor 1, antagonists & inhibitors, genetics, metabolism, ADP-Ribosylation Factors, Antigen Presentation, Blotting, Western, Cells, Cultured, Cytoplasm, Enzyme-Linked Immunosorbent Assay, Fluorescent Antibody Technique, Genetic Vectors, HIV Infections, immunology, virology, HIV-1, pathogenicity, HLA-A2 Antigen, Humans, Immunoprecipitation, Protein Binding, Protein Transport, T-Lymphocytes, Transcription Factor AP-1, nef Gene Products, Human Immunodeficiency Virus

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      Abstract

      HIV-1-infected cells are partially resistant to anti-HIV cytotoxic T lymphocytes (CTLs) due to the effects of the HIV Nef protein on antigen presentation by major histocompatibility complex class I (MHC-I), and evidence has been accumulating that this function of Nef is important in vivo. HIV Nef disrupts the normal expression of MHC-I by stabilizing a protein-protein interaction between the clathrin adaptor protein AP-1 and the MHC-I cytoplasmic tail. There is also evidence that Nef activates a phosphatidylinositol 3 kinase (PI3K)-dependent GTPase, ADP ribosylation factor 6 (ARF-6), to stimulate MHC-I internalization. However, the relative importance of these two pathways is unclear. Here we report that a GTPase required for AP-1 activity (ARF-1) was needed for Nef to disrupt MHC-I surface levels, whereas no significant requirement for ARF-6 was observed in Nef-expressing T cell lines and in HIV-infected primary T cells. An ARF-1 inhibitor blocked the ability of Nef to recruit AP-1 to the MHC-I cytoplasmic tail, and a dominant active ARF-1 mutant stabilized the Nef-MHC-I-AP-1 complex. These data support a model in which Nef and ARF-1 stabilize an interaction between MHC-I and AP-1 to disrupt the presentation of HIV-1 epitopes to CTLs.

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      Journal
      10.1128/JVI.00056-11
      3209341
      21917951

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