Corrigendum to “Multicentre Harmonisation of a Six-Colour Flow Cytometry Panel for Naïve/Memory T Cell Immunomonitoring”

There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

Abstract

In the article titled “Multicentre Harmonisation of a Six-Colour Flow Cytometry Panel for Naïve/Memory T Cell Immunomonitoring” [1], there was an error in Table 1. The table should show affiliation of one of the two BD LSR Fortessa instruments used in the study and cited in Table 1. The corrected table is shown below and is listed as Table 1.

Related collections

Most cited references 1

Record: found
Abstract: found
Article: found

Is Open Access

Multicentre Harmonisation of a Six-Colour Flow Cytometry Panel for Naïve/Memory T Cell Immunomonitoring

Iole Macchia, Valentina La Sorsa, Irene Ruspantini … (2020)

Background Personalised medicine in oncology needs standardised immunological assays. Flow cytometry (FCM) methods represent an essential tool for immunomonitoring, and their harmonisation is crucial to obtain comparable data in multicentre clinical trials. The objective of this study was to design a harmonisation workflow able to address the most effective issues contributing to intra- and interoperator variabilities in a multicentre project. Methods The Italian National Institute of Health (Istituto Superiore di Sanità, ISS) managed a multiparametric flow cytometric panel harmonisation among thirteen operators belonging to five clinical and research centres of Lazio region (Italy). The panel was based on a backbone mixture of dried antibodies (anti-CD3, anti-CD4, anti-CD8, anti-CD45RA, and anti-CCR7) to detect naïve/memory T cells, recognised as potential prognostic/predictive immunological biomarkers in cancer immunotherapies. The coordinating centre distributed frozen peripheral blood mononuclear cells (PBMCs) and fresh whole blood (WB) samples from healthy donors, reagents, and Standard Operating Procedures (SOPs) to participants who performed experiments by their own equipment, in order to mimic a real-life scenario. Operators returned raw and locally analysed data to ISS for central analysis and statistical elaboration. Results Harmonised and reproducible results were obtained by sharing experimental set-up and procedures along with centralising data analysis, leading to a reduction of cross-centre variability for naïve/memory subset frequencies particularly in the whole blood setting. Conclusion Our experimental and analytical working process proved to be suitable for the harmonisation of FCM assays in a multicentre setting, where high-quality data are required to evaluate potential immunological markers, which may contribute to select better therapeutic options.

0 comments Cited 7 times – based on 0 reviews      Review now

Bookmark

All references

Author and article information

Contributors

Iole Macchia:

ORCID: https://orcid.org/0000-0002-3870-8202

Francesca Urbani:

ORCID: https://orcid.org/0000-0002-5522-2706

Journal

Journal ID (nlm-ta): J Immunol Res

Journal ID (iso-abbrev): J Immunol Res

Journal ID (publisher-id): JIR

Title: Journal of Immunology Research

Publisher: Hindawi

ISSN (Print): 2314-8861

ISSN (Electronic): 2314-7156

Publication date Collection: 2020

Publication date (Electronic): 10 August 2020

Publication date PMC-release: 10 August 2020

Volume: 2020

Electronic Location Identifier: 2698258

Affiliations

¹Department of Oncology and Molecular Medicine, Istituto Superiore di Sanità (ISS), Rome 00161, Italy

²Research Coordination and Support Service, CoRI, ISS, Rome 00161, Italy

³Core Facilities, ISS, Rome 00161, Italy

⁴Department of Infectious Diseases, ISS, Rome 00161, Italy

⁵Center for Gender-Specific Medicine, ISS, Rome 00161, Italy

⁶Unit of Tumor Immunology and Immunotherapy, IRCCS Regina Elena National Cancer Institute (IRE), Rome 00128, Italy

⁷Department of Experimental Medicine, Sapienza University of Rome (SUR), 00185, Italy

⁸Department of Molecular Medicine, SUR, Rome 00185, Italy

⁹Clinical Research, Imperial College, London W12 ONN, UK

¹⁰Onco-Hematology Department, IRCCS Bambino Gesù Children's Hospital (OPBG), Rome 00165, Italy

¹¹Cellular Immunology Laboratory, IRCCS National Institute for Infectious Diseases “L. Spallanzani” (INMI), Rome 00149, Italy

¹²Institute of Translational Pharmacology, National Research Council, Rome 00185, Italy

¹³Medical Biotechnology and Translational Medicine PhD School, Tor Vergata University, Rome 00133, Italy

Author information

Iole Macchia https://orcid.org/0000-0002-3870-8202

Valentina La Sorsa https://orcid.org/0000-0002-4141-7561

Giorgio Fedele https://orcid.org/0000-0003-0017-6711

Giovanna Schiavoni https://orcid.org/0000-0001-9135-0556

Paola Rizza https://orcid.org/0000-0002-4980-3010

Paola Nisticò https://orcid.org/0000-0003-4409-2261

Belinda Palermo https://orcid.org/0000-0001-6293-0113

Stefania Morrone https://orcid.org/0000-0003-2441-9749

Aurelia Rughetti https://orcid.org/0000-0002-1863-8251

Marianna Nuti https://orcid.org/0000-0003-1809-1003

Ilaria Grazia Zizzari https://orcid.org/0000-0001-8263-7248

Cinzia Fionda https://orcid.org/0000-0003-0636-7607

Roberta Maggio https://orcid.org/0000-0001-8319-4418

Cristina Capuano https://orcid.org/0000-0002-6747-3836

Matilde Sinibaldi https://orcid.org/0000-0001-5277-8998

Chiara Agrati https://orcid.org/0000-0002-5252-0927

Francesca Urbani https://orcid.org/0000-0002-5522-2706

Article

DOI: 10.1155/2020/2698258

PMC ID: 7436290

SO-VID: c807cdff-4cdc-40cc-b48b-0b19ab5ac356

License:

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Corrigendum to “Multicentre Harmonisation of a Six-Colour Flow Cytometry Panel for Naïve/Memory T Cell Immunomonitoring”

Read this article at

Abstract

Related collections

Embodied Memory

Most cited references 1

Multicentre Harmonisation of a Six-Colour Flow Cytometry Panel for Naïve/Memory T Cell Immunomonitoring

Author and article information

Contributors

Journal

Affiliations

Author information

Article

History

Categories

Comments

Comment on this article

Similar content 9

Most referenced authors 31