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      Two-dimensional map of human brain proteins.

      Electrophoresis

      Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Animals, Rats, analysis, Proteins, Peptide Mapping, chemistry, Parietal Lobe, Middle Aged, Mice, Humans, Electrophoresis, Gel, Two-Dimensional, Cattle, Brain Chemistry

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          Abstract

          Samples of human brain from the parietal cortex lobe were analyzed by two-dimensional gel electrophoresis, using immobilized pH gradient strips covering the various pH regions. The protein spots were visualized with colloidal Coomassie blue stain and identified by matrix-assisted laser desorption/ionization mass spectrometry. Approximately 400 spots were identified, corresponding to 180 different brain proteins. The list of identified proteins includes a large number of structural proteins and of enzymes or enzyme subunits with various catalytic activities. The majority of proteins are localized in the cytoplasma and in mitochondria. The two-dimensional map may be useful as a reference database to study changes in the protein level caused by various disorders, such as Alzheimer's disease, major depression and schizophrenia.

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          Author and article information

          Journal
          10344266
          10.1002/(SICI)1522-2683(19990101)20:4/5<907::AID-ELPS907>3.0.CO;2-A

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