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The analysis of café espresso using two-dimensional reversed phase–reversed phase high performance liquid chromatography with UV-absorbance and chemiluminescence detection
Mariam Mnatsakanyan ,
Paul G. Stevenson ,
Xavier A. Conlan ,
Paul S. Francis ,
Tiffany A. Goodie ,
Geoffrey P. McDermott ,
Neil W. Barnett ,
R. Andrew Shalliker
September 15 2010
September 15 2010
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Abstract
In this study, an activity based screening technique combining two-dimensional liquid
chromatography (2DHPLC) with UV-absorbance and chemiluminescence detection was applied
to study "Ristretto", "Decaffeinatto" and "Volluto" espresso coffees. This technique,
which coupled the separation power of 2DHPLC with the sensitivity and selectivity
of the chemiluminescence detection, offers great potential for screening complex samples
for antioxidant compounds. Detailed information regarding the complexity of the sample,
and the variation between these three coffees could be obtained using this multidimensional-hyphenated
method of analysis.
Copyright (c) 2010 Elsevier B.V. All rights reserved.