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      First subtyping of Blastocystis sp. from pet rodents in southwestern China

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          Abstract

          Blastocystis sp. is a common eukaryotic parasite, which infects humans as well as various other animals. To date, epidemiological data regarding the detection rate and distribution of Blastocystis sp. subtypes in pet rodents are lacking in China; the present study aims to fill this gap. A total of 503 fecal samples collected from pets in different locations in southwestern China were screened for the presence of Blastocystis sp. using a nested PCR amplification of SSU rRNA method. Forty-two samples (8.35%) tested positive for Blastocystis sp. colonization. Two subtypes of Blastocystis sp. were identified based on nucleotide sequence homology and phylogenetic analysis: Blastocystis ST4 was present in 41 samples, and Blastocystis ST17 was found in 1 sample. Our results revealed robust host preference of Blastocystis ST4 and confirmed that Blastocystis ST17 can also parasitize rodents.

          Highlights

          • •

            This is the first survey of Blastocystis sp. from pet rodent in southwestern of China and that 503 rodents were surveyed.

          • •

            Forty-two samples (8.35%) tested positive for Blastocystis sp. colonization.

          • •

            Forty-one isolates were identified as subtype ST4 and one as ST17.

          • •

            Subtype ST4 shows a robust host preference for rodents.

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          New insights on classification, identification, and clinical relevance of Blastocystis spp.

          Kevin S W Tan (2008)
          Blastocystis is an unusual enteric protozoan parasite of humans and many animals. It has a worldwide distribution and is often the most commonly isolated organism in parasitological surveys. The parasite has been described since the early 1900s, but only in the last decade or so have there been significant advances in our understanding of Blastocystis biology. However, the pleomorphic nature of the parasite and the lack of standardization in techniques have led to confusion and, in some cases, misinterpretation of data. This has hindered laboratory diagnosis and efforts to understand its mode of reproduction, life cycle, prevalence, and pathogenesis. Accumulating epidemiological, in vivo, and in vitro data strongly suggest that Blastocystis is a pathogen. Many genotypes exist in nature, and recent observations indicate that humans are, in reality, hosts to numerous zoonotic genotypes. Such genetic diversity has led to a suggestion that previously conflicting observations on the pathogenesis of Blastocystis are due to pathogenic and nonpathogenic genotypes. Recent epidemiological, animal infection, and in vitro host-Blastocystis interaction studies suggest that this may indeed be the case. This review focuses on such recent advances and also provides updates on laboratory and clinical aspects of Blastocystis spp.
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            DNA barcoding of blastocystis.

            Kaitlin C Clark, Blessing Tawari, M. T. Scicluna (2006)
            We have developed a simple method for subtyping the intestinal protistan parasite Blastocystis using an approach equivalent to DNA barcoding in animals. Amplification of a 600 bp region of the small subunit ribosomal RNA gene followed by single primer sequencing of the PCR product provides enough data to assign isolates to specific subtypes unambiguously. We believe that this approach will prove useful in future epidemiological studies.
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              Genetic diversity of blastocystis in livestock and zoo animals.

              Kaitlin C Clark, Mohammed A Alfellani, Reeba Jacob … (2013)
              Blastocystis is a common unicellular anaerobic eukaryote that inhabits the large intestine of many animals worldwide, including humans. The finding of Blastocystis in faeces in mammals and birds has led to proposals of zoonotic potential and that these hosts may be the source of many human infections. Blastocystis is, however, a genetically diverse complex of many distinct organisms (termed subtypes; STs), and sampling to date has been limited, both geographically and in the range of hosts studied. In order to expand our understanding of host specificity of Blastocystis STs, 557 samples were examined from various non-primate animal hosts and from a variety of different countries in Africa, Asia and Europe. STs were identified using 'barcoding' of the small subunit rRNA gene using DNA extracted either from culture or directly from faeces. The host and geographic range of several STs has thereby been greatly expanded and the evidence suggests that livestock is not a major contributor to human infection. Two new STs were detected among the barcode sequences obtained; for these, and for three others where the data were incomplete, the corresponding genes were fully sequenced and phylogenetic analysis was undertaken. Copyright © 2013 Elsevier GmbH. All rights reserved.
              Article 0 comments Cited 133 times – based on 0 reviews     Review now
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                Author and article information

                Contributors
                Guangneng Peng
                Journal
                Journal ID (nlm-ta): Int J Parasitol Parasites Wildl
                Journal ID (iso-abbrev): Int J Parasitol Parasites Wildl
                Title: International Journal for Parasitology: Parasites and Wildlife
                Publisher: Elsevier
                ISSN (Electronic): 2213-2244
                Publication date PMC-release: 30 January 2020
                Publication date Collection: April 2020
                Publication date (Electronic): 30 January 2020
                Volume: 11
                Pages: 143-148
                Affiliations
                [1]The Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China
                Author notes
                [∗ ]Corresponding author. pgn.sicau@ 123456163.com
                [1]

                These authors have contributed equally to this work.

                Article
                Publisher ID: S2213-2244(20)30012-2
                DOI: 10.1016/j.ijppaw.2020.01.012
                PMC ID: 7011001
                PubMed ID: 32071861
                SO-VID: c8c2d0e9-f653-46b7-a521-a1d8f0ad790c
                Copyright © © 2020 The Author(s)
                License:

                This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

                History
                Date received : 18 September 2019
                Date revision received : 24 January 2020
                Date accepted : 25 January 2020
                Categories
                Subject: Article

                Keywords: blastocystis sp.,pet rodent,subtype,china
                Data availability:
                Keywords: blastocystis sp., pet rodent, subtype, china

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