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      Inhibition of PCR-based assay for Bordetella pertussis by using calcium alginate fiber and aluminum shaft components of a nasopharyngeal swab.

      Journal of Clinical Microbiology
      Alginates, Aluminum, Bacteriological Techniques, instrumentation, Base Sequence, Bordetella pertussis, genetics, isolation & purification, DNA Probes, DNA, Bacterial, Glucuronic Acid, Hexuronic Acids, Humans, Molecular Sequence Data, Nasopharynx, microbiology, Polyethylene Terephthalates, Polymerase Chain Reaction, Whooping Cough, diagnosis

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          Abstract

          A PCR-based assay for Bordetella pertussis was inhibited by using a calcium alginate fiber-tipped swab with an aluminum shaft but not by using a Dacron fiber-tipped swab with a plastic shaft. The calcium alginate fiber component inhibited the assay following storage for less than 1 min in a suspension of 10(3) CFU of B. pertussis per ml, whereas the aluminum shaft component required storage for at least 48 h in order to cause inhibition. We recommend the Dacron swab over the calcium alginate swab for collecting specimens for testing in PCR-based assays.

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