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      Delivery methods for CRISPR/Cas9 gene editing in crustaceans

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          Abstract

          In this mini-review, we provide an up-to-date overview of the delivery methods that have been used for CRISPR/Cas9 genomic editing in crustacean species. With embryonic microinjection as the main workforce for delivering CRISPR/Cas9 reagents, biologists working with crustacean species have to tackle the technical challenges involved in microinjection. We use examples of three crustacean species (the branchiopod Daphnia, amphipod Parhyale hawaiensis, and decapod Exopalaemon carinicauda) to provide a technical guide for embryonic microinjection. Moreover, we outline two potentially useful new techniques for delivering CRISPR/Cas9 components into crustaceans, i.e., Receptor-Mediated Ovary Transduction of Cargo (ReMOT Control) and electroporation.

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          Author and article information

          Journal
          MLST
          Marine Life Science & Technology
          Springer (China )
          2096-6490
          2662-1746
          01 February 2020
          14 November 2019
          : 2
          : 1
          : 1-5
          Affiliations
          1Department of Biology, University of Texas at Arlington, 501 S. Nedderman Dr, Arlington, TX 76019, USA
          Author notes
          *Corresponding author: Sen Xu, E-mail: sen.xu@ 123456uta.edu
          Article
          s42995-019-00011-4
          10.1007/s42995-019-00011-4
          © 2019 Ocean University of China
          Product
          Self URI (journal-page): https://www.springer.com/journal/42995
          Categories
          Review

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