156
views
0
recommends
+1 Recommend
1 collections
    2
    shares
      • Record: found
      • Abstract: found
      • Article: found

      Delivery methods for CRISPR/Cas9 gene editing in crustaceans

      review-article

      Read this article at

      ScienceOpenPublisherPMC
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          In this mini-review, we provide an up-to-date overview of the delivery methods that have been used for CRISPR/Cas9 genomic editing in crustacean species. With embryonic microinjection as the main workforce for delivering CRISPR/Cas9 reagents, biologists working with crustacean species have to tackle the technical challenges involved in microinjection. We use examples of three crustacean species (the branchiopod Daphnia, amphipod Parhyale hawaiensis, and decapod Exopalaemon carinicauda) to provide a technical guide for embryonic microinjection. Moreover, we outline two potentially useful new techniques for delivering CRISPR/Cas9 components into crustaceans, i.e., Receptor-Mediated Ovary Transduction of Cargo (ReMOT Control) and electroporation.

          Related collections

          Author and article information

          Journal
          MLST
          Marine Life Science & Technology
          Springer (China )
          2096-6490
          2662-1746
          01 February 2020
          14 November 2019
          : 2
          : 1
          : 1-5
          Affiliations
          [1] 1Department of Biology, University of Texas at Arlington, 501 S. Nedderman Dr, Arlington, TX 76019, USA
          Author notes
          *Corresponding author: Sen Xu, E-mail: sen.xu@ 123456uta.edu
          Article
          s42995-019-00011-4
          10.1007/s42995-019-00011-4
          7731668
          33313574
          c8dfdfd2-56cb-4999-862c-30fb589d68ac
          © 2019 Ocean University of China
          History
          : 30 June 2019
          : 12 September 2019
          Categories
          Review

          Evolutionary Biology,Cell biology,Aquaculture & Fisheries,Ecology,Biotechnology,Life sciences
          Electroporation,Vitellogenesis, Parhyale hawaiensis , Exopalaemon carinicauda , Daphnia ,Microinjection

          Comments

          Comment on this article