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Using a set of synthetic oligonucleotides homologous to broadly conserved sequences
in-vitro amplification via the polymerase chain reaction followed by direct sequencing
results in almost complete nucleotide determination of a gene coding for 16S ribosomal
RNA. As a model system the nucleotide sequence of the 16S rRNA gene of M.kansasii
was determined and found to be 98.7% homologous to that of M.bovis BCG. This is the
first report on a contiguous sequence information of an entire amplified gene spanning
1.5 kb without any subcloning procedures.