Blog
About

43
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: not found
      • Article: not found

      Isolation and direct complete nucleotide determination of entire genes. Characterization of a gene coding for 16S ribosomal RNA

      , , , ,

      Nucleic Acids Research

      Oxford University Press (OUP)

      Read this article at

      ScienceOpenPublisherPMC
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Using a set of synthetic oligonucleotides homologous to broadly conserved sequences in-vitro amplification via the polymerase chain reaction followed by direct sequencing results in almost complete nucleotide determination of a gene coding for 16S ribosomal RNA. As a model system the nucleotide sequence of the 16S rRNA gene of M.kansasii was determined and found to be 98.7% homologous to that of M.bovis BCG. This is the first report on a contiguous sequence information of an entire amplified gene spanning 1.5 kb without any subcloning procedures.

          Related collections

          Author and article information

          Journal
          Nucleic Acids Research
          Nucl Acids Res
          Oxford University Press (OUP)
          0305-1048
          1362-4962
          1989
          1989
          : 17
          : 19
          : 7843-7853
          Article
          10.1093/nar/17.19.7843
          334891
          2798131
          © 1989

          Comments

          Comment on this article