Real-Time PCR Quantification of AT1 and AT2 Angiotensin Receptor mRNA Expression in the Developing Rat Kidney

Abstract

Background/Aims: Angiotensin II (Ang II) is an important growth factor in the fetal kidney. Molecular cloning and pharmacological studies have defined two major classes of Ang II receptors designated AT1 and AT2. Two AT1 isoforms, AT1A and AT1B, exist in rodents. AT1 promotes growth and proliferation and mediates many of the known physiological actions of Ang II. AT2 appears to antagonize AT1. Our goal was to measure their relative contributions to Ang II signaling in the developing kidney. Methods: We used real-time RT-PCR to quantify AT1A, AT1B, AT2 and the housekeeping gene EF1α mRNA in kidneys from embryonic (E) day 14–20 and postnatal (P) day 1–14 rats. Results: AT2 mRNA declined from 1.4 × 10⁴ copies/10⁶ copies EF1α on E14 to 4.2 × 10³ copies/10⁶ copies EF1α on P14. In contrast, total AT1 mRNA increased gradually from 2.0 × 10³ copies/10⁶ copies EF1α on E14 to 2.0 × 10⁴ copies/10⁶ copies EF1α on P14, with AT1A accounting for about 90% of total AT1 mRNA throughout nephrogenesis. Moreover, the ratio of AT2/(AT1A + AT1B) decreased in a log-linear fashion during maturation, from 6.7 on E14 to 0.2 on P14. Conclusion: The ratio of AT2 to AT1 gene expression modulates Ang II action in the developing kidney.

Journal

Journal ID (publisher-id): NEE

Journal ID (nlm-ta): Nephron Exp Nephrol

Journal ID (doi): 10.1159/issn.1660-2129

Title: Cardiorenal Medicine

Publisher: S. Karger AG

ISSN (Electronic): 1660-2129

Publication date Subscription-year: 2003

Publication date (Print): August 2003

Publication date (Electronic): 17 November 2004

Volume: 94

Issue: 4

Pages: e154-e159

Affiliations

Departments of ^aPediatrics, ^bPathology, ^cNeurobiology and Anatomy and ^dChildren’s Health Research Center, University of Utah School of Medicine, Salt Lake City, Utah, USA

Article

Publisher ID: 72499 Other ID: Nephron Exp Nephrol 2003;94:e154–e159

DOI: 10.1159/000072499

PubMed ID: 12972714

Copyright statement: © 2003 S. Karger AG, Basel

License:

Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

Page count

Figures: 3, References: 47, Pages: 1

Product

Self URI (application/pdf): https://www.karger.com/Article/Pdf/72499

Self URI (text/html): https://www.karger.com/Article/FullText/72499

Self URI (journal page): https://www.karger.com/SubjectArea/Nephrology

Categories

Subject: Original Paper