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      A fraction of the mouse genome that is derived from islands of nonmethylated, CpG-rich DNA

      , , , ,

      Cell

      Elsevier BV

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          Most cited references 27

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          [57] Sequencing end-labeled DNA with base-specific chemical cleavages

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            The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.

             J Messing,  J. Vieira (1982)
            A series of plasmid vectors containing the multiple cloning site (MCS7) of M13mp7 has been constructed. In one of these vectors a kanamycin-resistance marker has been inserted into the center of the symmetrical MCS7 to yield a restriction-site-mobilizing element (RSM). The drug-resistance marker can be cleaved out of this vector with any of the restriction enzymes that recognize a site of the flanking sequences of the RSM to generate an RSM with either various sticky ends or blunt ends. These fragments can be used for insertion mutagenesis of any target molecule with compatible restriction sites. Insertion mutants are selected by their resistance to kanamycin. When the drug-resistance marker is removed with PstI, a small in-frame insertion can be generated. In addition, two new MCSs having single restriction sites have been formed by altering the symmetrical structure of MCS7. The resulting plasmids pUC8 and pUC9 allow one to clone doubly digested restriction fragments separately with both orientations in respect to the lac promoter. The terminal sequences of any DNA cloned in these plasmids can be characterized using the universal M13 primers.
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              DNA methylation and the frequency of CpG in animal DNA.

               Gregory Bird (1980)
              An analysis of nearest neighbour dinucleotide frequencies and the level of DNA methylation in animals strongly supports the suggestion that 5-methylcytosine (5mC) tends to mutate abnormally frequently to T. This tendency is the likely cause of the CpG deficiency in heavily methylated genomes.
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                Author and article information

                Journal
                Cell
                Cell
                Elsevier BV
                00928674
                January 1985
                January 1985
                : 40
                : 1
                : 91-99
                Article
                10.1016/0092-8674(85)90312-5
                © 1985

                http://www.elsevier.com/tdm/userlicense/1.0/

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