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      Reciprocal immuno-biological alterations occur during the co-culture of natural killer cells and adipose tissue-derived mesenchymal stromal cells

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          Abstract

          Due to their immune-therapeutic value, adipose tissue-derived mesenchymal stromal cells (AT-MSCs) require a better characterization of their interplay with natural killer (NK) cells known to contribute to the graft-versus-leukemia effects. When cultivated together, AT-MSCs showed cellular cytotoxicity and were therefore killed by NK cells in an activating-cytokine dependent manner. In the presence of AT-MSCs, both ligands and receptors known to drive NK cell interactions were significantly altered. During this co-culture, the proliferation of NK cells was slightly reduced, while their IFN-γ and TNF-α secretion was significantly increased. NK cells displayed sustained degranulation accompanied by increased discharge of their cytolytic granules (perforin, granzymes A and B). On the other hand, activated NK cells reduced the expression of serpins C1 and B9 in AT-MSCs. Collectively, reciprocal immuno-biological alterations occur during the co-culture of NK cells and AT-MSCs. Understanding these changes will increase the safety and efficacy of cell-based immuno-oncotherapy.

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          Author and article information

          Contributors
          mnajar@ulb.ac.be
          0032-474070772 , mfayyadk@ulb.ac.be , mfayyadk@gmail.com
          makram.merimi@gmail.com
          nathalie.meuleman@bordet.be
          Dominique.Bron@ulb.ac.be
          hfayyadk@gmail.com
          laurence.lagneaux@bordet.be
          Journal
          Cytotechnology
          Cytotechnology
          Cytotechnology
          Springer Netherlands (Dordrecht )
          0920-9069
          1573-0778
          10 January 2019
          February 2019
          : 71
          : 1
          : 375-388
          Affiliations
          [1 ] ISNI 0000 0001 2348 0746, GRID grid.4989.c, Laboratory of Clinical Cell Therapy, Institut Jules Bordet, , Université Libre de Bruxelles (ULB), ; Campus Erasme, Brussels, Belgium
          [2 ] ISNI 0000 0001 0743 2111, GRID grid.410559.c, Osteoarthritis Research Unit, , University of Montreal Hospital Research Center (CRCHUM), ; 900 Saint-Denis, R11.424, Montreal, QC H2X 0A9 Canada
          [3 ] ISNI 0000 0001 2348 0746, GRID grid.4989.c, Hematology Department, Institut Jules Bordet, , Université Libre de Bruxelles, ; 121 Boulevard de Waterloo, 1000 Brussels, Belgium
          [4 ] ISNI 0000 0004 1772 8348, GRID grid.410890.4, Laboratory of Physiology, Genetics and Ethnopharmacology, Faculty of Sciences, , University Mohammed Premier, ; Oujda, Morocco
          [5 ] ISNI 0000 0001 2324 3572, GRID grid.411324.1, Laboratory of Cancer Biology and Molecular Immunology, Faculty of Sciences I, , Lebanese University, ; Hadath, Lebanon
          Article
          PMC6368507 PMC6368507 6368507 294
          10.1007/s10616-019-00294-6
          6368507
          30632032
          ca017f95-756b-4c47-9e92-404783e06d7f
          © Springer Nature B.V. 2019
          History
          : 20 May 2018
          : 7 January 2019
          Categories
          Original Article
          Custom metadata
          © Springer Nature B.V. 2019

          Cell crosstalk,Immunomodulation,NK cells,AT-MSCs
          Cell crosstalk, Immunomodulation, NK cells, AT-MSCs

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