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      ELISA determination of soluble urokinase receptor in blood from healthy donors and cancer patients.

      Clinical chemistry
      Adult, Aged, Aged, 80 and over, Blood Donors, Enzyme-Linked Immunosorbent Assay, Female, Humans, Male, Middle Aged, Neoplasms, blood, Receptors, Cell Surface, Receptors, Urokinase Plasminogen Activator, Urokinase-Type Plasminogen Activator, metabolism

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          Abstract

          Measurement of urokinase receptor (uPAR) in tumor extracts has prognostic value, but assay of the soluble uPAR (suPAR) in peripheral blood may offer wider applications in cancer patient management. A tumor extract uPAR ELISA was modified to eliminate nonspecific plasma protein interference, enabling specific detection of suPAR in plasma and sera with >90% recovery of added calibrator. suPAR concentrations in citrate plasma correlated with sera in 93 healthy blood donors (r = 0.84, P <0.0001), with a median value for both of 1.2 microg/L. The plasma median for 19 advanced breast cancer patients was 2.9 microg/L suPAR, and a similar increase was found for 10 advanced colon cancer patients, consistent with release of suPAR from tumors into blood. Repetitive monitoring of suPAR in cancer patients' blood may have value in assessment of prognosis and tumor recurrence.

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