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      Simultaneous analysis of adenosine triphosphate and its metabolites in Saccharomyces Cerevisiae using RP-HPLC

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          Abstract

          In order to assess the contribution of adenosine triphosphate and its metabolites to the cellular metabolism process in Saccharomyces cerevisiae, it is very important to simultaneously determine the relative concentrations of ATP and its metabolites. In this study, a fast, simple reversed-phase high-performance liquid chromatography with high selectivity was developed to simultaneously measure adenosine triphosphate and its metabolites (adenosine diphosphate, adenosine monophosphate, and cyclic adenosine monophosphate) in yeast. The method was performed under the gradient grogram, and the detection was monitored at 254 nm. Analysis was achieved within 25 min. The four components can be detected with linear response over the concentration range from 1 to 100 mg L −1 with excellent correlation coefficients ( r 2) > 0.999. The recovery of the four analytes was 92.9%, 90.4%, 99.1%, and 105.1%, respectively. To demonstrate the good analysis of yeast samples, changes in the four adenine nucleotides levels caused by caloric restriction in yeast were determined. It is expected that the current method may contribute to further metabolomics and system biology investigations of yeast.

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          Author and article information

          Contributors
          Journal
          1326
          Acta Chromatographica
          Acta Chromatographica
          Akadémiai Kiadó
          1233-2356
          December 2016
          : 28
          : 4
          : 501-511
          Affiliations
          [1]State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology , 130 Meilong Road, Shanghai 200237, China
          Author notes
          Article
          10.1556/1326.2016.28.4.10
          ca77d1d2-fcad-4b10-8261-cb179bdc51b4
          © The Author(s)

          This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium for non-commercial purposes, provided the original author and source are credited.

          History
          : 9 February 2016
          Page count
          Pages: 11
          Categories
          Original Research Paper

          Materials properties,Nanomaterials,Chemistry,Nanotechnology,Analytical chemistry,Thin films & surfaces
          adenosine,adenosine triphosphate,monophosphate,RP-HPLC,adenosine diphosphate,cyclic adenosine monophosphate,yeast

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