Quality assurance is becoming increasingly important. Good laboratory practice (GLP)
and good manufacturing practice (GMP) are now established standards. The biomedical
field aims at an increasing reliance on the use of in vitro methods. Cell and tissue
culture methods are generally fast, cheap, reproducible and reduce the use of experimental
animals. Good cell culture practice (GCCP) is an attempt to develop a common standard
for in vitro methods. The implementation of the use of chemically defined media is
part of the GCCP. This will decrease the dependence on animal serum, a supplement
with an undefined and variable composition. Defined media supplements are commercially
available for some cell types. However, information on the formulation by the companies
is often limited and such supplements can therefore not be regarded as completely
defined. The development of defined media is difficult and often takes place in isolation.
A workshop was organised in 2009 in Copenhagen to discuss strategies to improve the
development and use of serum-free defined media. In this report, the results from
the meeting are discussed and the formulation of a basic serum-free medium is suggested.
Furthermore, recommendations are provided to improve information exchange on newly
developed serum-free media.
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