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      Acheate-scute like 1 (Ascl1) is required for normal delta-like (Dll) gene expression and notch signaling during retinal development.

      Developmental Dynamics
      Animals, Basic Helix-Loop-Helix Transcription Factors, genetics, metabolism, physiology, Calcium-Binding Proteins, Cell Differentiation, Eye, embryology, Gene Expression Regulation, Developmental, In Situ Hybridization, Intercellular Signaling Peptides and Proteins, Intracellular Signaling Peptides and Proteins, Membrane Proteins, Mice, Mice, Inbred C57BL, Mice, Knockout, Nerve Tissue Proteins, Neurons, cytology, Polymerase Chain Reaction, Receptors, Notch, Repressor Proteins, Retina, Signal Transduction, Stem Cells

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          Abstract

          Delta gene expression in Drosophila is regulated by proneural basic helix-loop-helix (bHLH) transcription factors, such as acheate-scute. In vertebrates, multiple Delta-like and proneural bHLH genes are expressed during neurogenesis, especially in the retina. We recently uncovered a relationship between Acheate-scute like 1 (Ascl1), Delta-like genes, and Notch in chick retinal progenitors. Here, we report that mammalian retinal progenitors are also the primary source of Delta-like genes, likely signaling through Notch among themselves, while differentiating neurons expressed Jagged2. Ascl1 is coexpressed in Delta-like and Notch active progenitors, and required for normal Delta-like gene expression and Notch signaling. We also reveal a role for Ascl1 in the regulation of Hes6, a proneurogenic factor that inhibits Notch signaling to promote neural rather than glial differentiation. Thus, these results suggest a molecular mechanism whereby attenuated Notch levels coupled with reduced proneurogenic activity in progenitors leads to increased gliogenesis and decreased neurogenesis in the Ascl1-deficient retina. 2009 Wiley-Liss, Inc.

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