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      • Record: found
      • Abstract: found
      • Article: found

      Interaction between Phosphoinositide-Phospholipase C and Phosphatidylcholine- Phospholipase D in Plasma Membranes of Retinal Capillary Pericytes

      a,b , b
      Ophthalmic Research
      S. Karger AG
      Pericyte, Phosphatidylcholine, Phospholipase C, Phospholipase D

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          The existence of phosphatidylcholine-specifïc phospholipase D (PC-PLD) was demonstrated in the isolated plasma membranes of retinal capillary pericytes. We determined PC-PLD activity, taking advantage of the trans-phosphatidylation activity of PC-PLD in the presence of ethanol, with phosphatidylethanol (PEt) formation. In the membrane environment, both phosphoinositide-specific phospholipase C (PI-PLC) and PC-PLD were activated by GTPγS. Neomycin, an inhibitor of PI-PLC, at a concentration (500 μ M) capable of inhibiting 90% of the PI-PLC activity, only slightly inhibited the basal level of PC-PLD, but significantly decreased the GTPγS-activated PC-PLD by 66%. These findings indicate that the inhibitory effects of neomycin on PC hydrolysis may exert through the regulatory mechanisms of G proteins. Exogenous phosphatidic acid, a product of PC-PLD, stimulated, whereas PC, the substrate of PC-PLD, inhibited PI-PLC. These data implicate one mechanism by which PC-PLD may modulate PI-PLC activity through changing the phospholipid composition in the membrane, specifically the ratio of PA/PC. These findings are useful for the further studies on the communication between membrane-associated PI-PLC- and PC-PLD-mediated signal transduction pathways.

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          Author and article information

          Ophthalmic Res
          Ophthalmic Research
          S. Karger AG
          11 December 2009
          : 26
          : 3
          : 189-195
          aDepartment of Ophthalmology, Peking Union Medical College Hospital, Beijing, China; bHahnemann University, Philadelphia, Pa., USA
          267411 Ophthalmic Res 1994;26:189–195
          © 1994 S. Karger AG, Basel

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          : 28 October 1993
          : 21 December 1993
          Page count
          Pages: 7
          Original Paper

          Vision sciences,Ophthalmology & Optometry,Pathology
          Pericyte,Phospholipase D,Phospholipase C,Phosphatidylcholine


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