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      • Record: found
      • Abstract: found
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      Interaction between Phosphoinositide-Phospholipase C and Phosphatidylcholine- Phospholipase D in Plasma Membranes of Retinal Capillary Pericytes

      research-article
      a,b , b
      Ophthalmic Research
      S. Karger AG
      Pericyte, Phosphatidylcholine, Phospholipase C, Phospholipase D

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          Abstract

          The existence of phosphatidylcholine-specifïc phospholipase D (PC-PLD) was demonstrated in the isolated plasma membranes of retinal capillary pericytes. We determined PC-PLD activity, taking advantage of the trans-phosphatidylation activity of PC-PLD in the presence of ethanol, with phosphatidylethanol (PEt) formation. In the membrane environment, both phosphoinositide-specific phospholipase C (PI-PLC) and PC-PLD were activated by GTPγS. Neomycin, an inhibitor of PI-PLC, at a concentration (500 μ M) capable of inhibiting 90% of the PI-PLC activity, only slightly inhibited the basal level of PC-PLD, but significantly decreased the GTPγS-activated PC-PLD by 66%. These findings indicate that the inhibitory effects of neomycin on PC hydrolysis may exert through the regulatory mechanisms of G proteins. Exogenous phosphatidic acid, a product of PC-PLD, stimulated, whereas PC, the substrate of PC-PLD, inhibited PI-PLC. These data implicate one mechanism by which PC-PLD may modulate PI-PLC activity through changing the phospholipid composition in the membrane, specifically the ratio of PA/PC. These findings are useful for the further studies on the communication between membrane-associated PI-PLC- and PC-PLD-mediated signal transduction pathways.

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          Author and article information

          Journal
          ORE
          Ophthalmic Res
          10.1159/issn.0030-3747
          Ophthalmic Research
          S. Karger AG
          0030-3747
          1423-0259
          1994
          1994
          11 December 2009
          : 26
          : 3
          : 189-195
          Affiliations
          aDepartment of Ophthalmology, Peking Union Medical College Hospital, Beijing, China; bHahnemann University, Philadelphia, Pa., USA
          Article
          267411 Ophthalmic Res 1994;26:189–195
          10.1159/000267411
          8090436
          cace7ab7-b306-45b1-a3a1-fd4b8b5b5c14
          © 1994 S. Karger AG, Basel

          Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

          History
          : 28 October 1993
          : 21 December 1993
          Page count
          Pages: 7
          Categories
          Original Paper

          Vision sciences,Ophthalmology & Optometry,Pathology
          Pericyte,Phospholipase D,Phospholipase C,Phosphatidylcholine

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