12
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: not found

      Molecular reaction mechanisms of proteins monitored by time-resolved FTIR-spectroscopy.

      Biological chemistry
      Bacteriorhodopsins, chemistry, Oncogene Protein p21(ras), Spectroscopy, Fourier Transform Infrared

      Read this article at

      ScienceOpenPublisherPubMed
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Time-resolved FTIR difference spectroscopy can provide a valuable insight into the molecular reaction mechanisms of proteins, especially membrane proteins. Isotopic labeling and site-directed mutagenesis allows an unequivocal assignment of IR absorption bands. Studies are presented which give insight into the proton pump mechanisms of proteins, especially bacteriorhodopsin. H-bonded network proton transfer via internal water molecules seems to be a general feature in proteins, also found in cytochrome c oxidase. Using caged GTP the intrinsic and GAP catalyzed GTPase activity of H-ras p21 is studied. Furthermore, protein folding reactions can be recorded with ns time-resolution.

          Related collections

          Author and article information

          Journal
          10494844
          10.1515/BC.1999.115

          Chemistry
          Bacteriorhodopsins,chemistry,Oncogene Protein p21(ras),Spectroscopy, Fourier Transform Infrared

          Comments

          Comment on this article