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      Half Smooth Tongue Sole ( Cynoglossus semilaevis) Under Low Salinity Stress Can Change Hepatic igf2 Expression Through DNA Methylation

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          Abstract

          Salinity is a crucial environmental stress that severely affects fish growth and survival. Under environmental stress, DNA methylation plays an important role in gene expression and genome function. To better understand the epigenetic regulation mechanism of igf2 under low salinity stress, we analyzed the DNA methylation at 5’UTR, exon1, intron1, and exon2, and analyzed the relationship of DNA methylation with mRNA abundance as well as the special single CpG sites methylation patterns of igf2 in the liver of half smooth tongue sole under low salinity (15) for 0, 7, and 60 d. When exposed to low salinity, DNA methylation at 5’UTR and exon2 remained stable, while it was up-regulated firstly and then down-regulated at exon1 and intron1. Some single CpG sites of igf2 associated with low salinity, and most of these sites with significantly changed methylation levels ( P < 0.05) are located in intron1 area. The discrepant variation of single CpG sites methylation levels and igf2 expression further revealed that females and males showed different response to low salinity. Remarkably, the 38-CpG site of intron1 servers as a sexual marker. Additionally, our integrative analysis demonstrated that regional DNA of igf2 methylation had highly complex interplay on gene expression. The single CpG sites in intron1 were indispensable epigenetic markers under external environmental stress. Above all, to resist the low salinity stress, half smooth tongue sole liver can regulate the expression of igf2 through methylation of CpG sites in intron1.

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          Author and article information

          Journal
          JOUC
          Journal of Ocean University of China
          Science Press and Springer (China )
          1672-5182
          20 December 2019
          01 February 2020
          : 19
          : 1
          : 171-182
          Affiliations
          1Fishery College, the Key Laboratory of Mariculture of Ministry of Education, Ocean University of China, Qingdao 266003, China
          Author notes
          *Corresponding authors: HE Feng, E-mail: hefengouc@ 123456ouc.edu.cn ; WEN Haishen, E-mail: wenhaishen@ 123456ouc.edu.cn
          Article
          s11802-019-4237-3
          10.1007/s11802-019-4237-3
          Copyright © Ocean University of China, Science Press and Springer-Verlag GmbH Germany 2020.

          The copyright to this article, including any graphic elements therein (e.g. illustrations, charts, moving images), is hereby assigned for good and valuable consideration to the editorial office of Journal of Ocean University of China, Science Press and Springer effective if and when the article is accepted for publication and to the extent assignable if assignability is restricted for by applicable law or regulations (e.g. for U.S. government or crown employees).

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          Self URI (journal-page): https://www.springer.com/journal/11802

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