Enterocytozoon bieneusi, a microsporidian species, is a zoonotic pathogen found in both humans and animals. Here, we determined the prevalence, explored the different genotypes of E. bieneusi in wild rhesus macaques ( Macaca mulatta) (Hainan Island of China), and assessed their zoonotic potential.
We collected 173 fecal specimens from wild rhesus macaques living in Nanwan Monkey Island, Hainan, China. Subsequently, we identified and genotyped E. bieneusi using nested PCR analysis amplification of the internal transcribed spacer region (ITS) of the rRNA gene. Lastly, a neighbor-joining tree was built based on gene sequences from the ITS region of E. bieneusi.
Of the 173 specimens from wild rhesus macaques, 26 (15%) were infected with E. bieneusi. We identified six genotypes of E. bieneusi, of which five were known: PigEBITS7 ( n = 20), D ( n = 2), Type IV ( n = 1), Peru6 (n = 1), Henan-III (n = 1), and a novel genotype: HNM-IX ( n = 1). From the phylogenetic analysis, the six genotypes identified here were all clustered into zoonotic group 1.