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      Molecular Depiction of Thirteen Indian Toxic Plants with ITS Markers

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      Arab Journal of Forensic Sciences & Forensic Medicine
      Naif Arab University for Security Sciences

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          Abstract

          Plant identification is an overwhelming task due to different biological attributes and great diversity in plant species. In the absence of physical markers, molecular techniques have become useful for the identification of species of origin of medicinal plant seeds, pastes, and formulations of suspected plants. The ITS region of nuclear rRNA was amplified from thirteen different toxic plant species by using universal primer ITS 1 & 4. Nucleotide sequences of all selected plants were submitted in NCBI and accession numbers were acquired. The results of this study give accurate identification of thirteen plant species and proved the ITS region of 18s-26s nuclear ribosome to be an important tool for phylogenetic analysis and species identification of plants. The sequence was aligned with top matched reference sequence and presented in Clustal Omega software for making a phylogenetic neighbour tree. The significance of these findings is paramount in forensic toxicology scenarios especially when fragmentary plant material is found in the stomach/intestine and its morphological identification becomes impossible. In these circumstances, the PCR based molecular technique surely plays a significant role in solving complicated forensic cases.

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          Author and article information

          Journal
          Arab Journal of Forensic Sciences & Forensic Medicine
          AJFSFM
          Naif Arab University for Security Sciences
          16586794
          16586786
          December 15 2020
          September 07 2020
          : 2
          : 2
          : 159-169
          Article
          10.26735/YGUY5295
          cb868fca-4316-48ab-a96a-4ecb62f91511
          © 2020

          This work is licensed under Attribution-NonCommercial 4.0 International license. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc/4.0/

          History

          Entomology,Pathology,Forensic science,Criminology,Dentistry,Anthropology
          Entomology, Pathology, Forensic science, Criminology, Dentistry, Anthropology

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