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Characteristics of a human cell line transformed by DNA from human adenovirus type 5.

The Journal of General Virology

biosynthesis, Adenoviruses, Human, immunology, metabolism, Animals, Antigens, Neoplasm, analysis, Antigens, Viral, Cell Division, Cell Line, Cell Transformation, Neoplastic, DNA, Viral, Glycoproteins, Humans, Mice, Neoplasm Transplantation, Peptide Biosynthesis, Viral Proteins

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      Human embryonic kidney cells have been transformed by exposing cells to sheared fragments of adenovirus type 5 DNA. The transformed cells (designated 293 cells) exhibited many of the characteristics of transformation including the elaboration of a virus-specific tumour antigen. Analysis of the polypeptides synthesized in the 293 cells by labelling with 35S-methionine and SDS PAGE showed a variable pattern of synthesis, different in a number of respects from that seen in otheruman cells. On labelling the surface of cells by lactoperoxidase catalysed radio-iodination, the absence of a labelled polypeptide analogous to the 250 K (LETS) glycoprotein was noted. Hybridization of labelled cellular RNA with restriction fragments of adenovirus type 5 DNA indicated transcription of a portion of the adenovirus genome at the conventional left hand end.

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