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      Characteristics of a human cell line transformed by DNA from human adenovirus type 5.

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      The Journal of general virology
      Microbiology Society

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          Abstract

          Human embryonic kidney cells have been transformed by exposing cells to sheared fragments of adenovirus type 5 DNA. The transformed cells (designated 293 cells) exhibited many of the characteristics of transformation including the elaboration of a virus-specific tumour antigen. Analysis of the polypeptides synthesized in the 293 cells by labelling with 35S-methionine and SDS PAGE showed a variable pattern of synthesis, different in a number of respects from that seen in otheruman cells. On labelling the surface of cells by lactoperoxidase catalysed radio-iodination, the absence of a labelled polypeptide analogous to the 250 K (LETS) glycoprotein was noted. Hybridization of labelled cellular RNA with restriction fragments of adenovirus type 5 DNA indicated transcription of a portion of the adenovirus genome at the conventional left hand end.

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          Author and article information

          Journal
          J Gen Virol
          The Journal of general virology
          Microbiology Society
          0022-1317
          0022-1317
          Jul 1977
          : 36
          : 1
          Article
          10.1099/0022-1317-36-1-59
          886304
          cbd01e18-9536-4d9c-9deb-b51b71378c64
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