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      Electrophysiological Recordings from Gonadotrophs

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          Abstract

          The electrophysiological properties of gonadotrophs have been studied in vitro, using the ovine adenohypophyseal pars tuberalis as a naturally enriched source of this cell type. Trypsin-dispersed pars tuberalis cells maintained in primary tissue culture had a membrane potential of -72 ± 4 mV (mean ± SEM) and an input resistance of 314 ± 38 MΩ. Spontaneous action potentials were not observed; however, a single spike could be induced by depolarizing current injection. The hypophysiotrophic peptide gonadotrophin-releasing hormone (GnRH) increased membrane voltage fluctuations, but these fluctuations (± 5–10 mV) did not induce action potentials or changes in membrane potential or resistance. Power spectra obtained from analysis of this noise indicated that the fundamental event underlying GnRH action has a mean life-time of 38.4 ± 4.5 ms. The obervations that cells incubated in recording medium secreted luteinizing hormone in response to GnRH and that the GnRH-induced increase in voltage noise was inhibited by Ca<sup>2+</sup> channel antagonists support the hypotheses (1) that gonadotrophin secretion is initiated by GnRH-induced Ca<sup>2+</sup> channel activation and (2) that action potentials are not a prerequisite for gonadotrophin release.

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          Author and article information

          Journal
          NEN
          Neuroendocrinology
          10.1159/issn.0028-3835
          Neuroendocrinology
          S. Karger AG
          0028-3835
          1423-0194
          1985
          1985
          28 March 2008
          : 41
          : 3
          : 258-268
          Affiliations
          Department of Neuroendocrinology, AFRC Institute of Animal Physiology, Babraham, Cambridge, UK
          Article
          124186 Neuroendocrinology 1985;41:258–268
          10.1159/000124186
          2413385
          cbd8d964-b28b-4056-94ae-c665b31fead5
          © 1985 S. Karger AG, Basel

          Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

          History
          : 17 October 1984
          : 05 December 1984
          Page count
          Pages: 11
          Categories
          Original Paper

          Endocrinology & Diabetes,Neurology,Nutrition & Dietetics,Sexual medicine,Internal medicine,Pharmacology & Pharmaceutical medicine
          Neuropeptides,Pituitary cells,Luteinizing hormone,Intracellular recording,Hormone secretion,Pars tuberalis,Anterior pituitary,Ion channels,Gonadotrophs

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