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      Culture-independent characterization of the autochthonous gut microbiota of grouper Epinephelus coioides following the administration of probiotic Enterococcus faecium

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          Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA

          We describe a new molecular approach to analyzing the genetic diversity of complex microbial populations. This technique is based on the separation of polymerase chain reaction-amplified fragments of genes coding for 16S rRNA, all the same length, by denaturing gradient gel electrophoresis (DGGE). DGGE analysis of different microbial communities demonstrated the presence of up to 10 distinguishable bands in the separation pattern, which were most likely derived from as many different species constituting these populations, and thereby generated a DGGE profile of the populations. We showed that it is possible to identify constituents which represent only 1% of the total population. With an oligonucleotide probe specific for the V3 region of 16S rRNA of sulfate-reducing bacteria, particular DNA fragments from some of the microbial populations could be identified by hybridization analysis. Analysis of the genomic DNA from a bacterial biofilm grown under aerobic conditions suggests that sulfate-reducing bacteria, despite their anaerobicity, were present in this environment. The results we obtained demonstrate that this technique will contribute to our understanding of the genetic diversity of uncharacterized microbial populations.
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            The current status and future focus of probiotic and prebiotic applications for salmonids

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              Role of gastrointestinal microbiota in fish

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                Author and article information

                Journal
                Aquaculture International
                Aquacult Int
                Springer Science and Business Media LLC
                0967-6120
                1573-143X
                August 2012
                February 9 2012
                August 2012
                : 20
                : 4
                : 791-801
                Article
                10.1007/s10499-012-9503-y
                cc102390-838f-4050-ac27-af13203e0d54
                © 2012

                http://www.springer.com/tdm

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