We report a list of anurans from the Reserva Biológica do Parazinho (00°52′30″ N, 49°59′15″ W), a strictly protected area, part of the Bailique archipelago, in the municipality of Macapá, state of Amapá, Brazil. The study was carried out in September–October 2010 (dry season) and May–June 2011 (rainy season). We recorded 13 species of anurans, distributed among four families: Bufonidae (1), Hylidae (8), Leptodactylidae (3) and Pipidae (1). The accumulation curves did not stabilize, suggesting that the community was not completely sampled. This study provides the first information on anurans in insular areas of the state of Amapá, and highlights the importance of these areas for the conservation of anurans.